TEMPORARY VENTRICULAR ASSISTANCE W/ PORCINE CARDIAC XENOGRAFTS

使用猪心脏异种移植物进行临时心室辅助

• 批准号: 6277814
• 负责人: DAVID H ADAMS
• 金额: $ 7.56万
• 依托单位: HARVARD MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-05-01 至 1999-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6277814
• 关键词: Mammalia; Primates; cardiovascular system; orphan disease /drug; surgery; transplantation; urinary tract

Post-cardiotomy cardiogenic shock is a significant clinical problem that will effect approximately 1% of all patients undergoing cardiac surgery this year. Experience with mechanical ventricular assistance in these patients at the Brigham and Women's Hospital as well as nationally has been very disappointing, and only approximately 25% of such patients supported with conventional technology will survive to discharge. We are very interested in pursuing the possibility of using genetically modified porcine xenografts to provide temporary cardiac support. Thus far, we have performed transplant surgeries on 11 baboons. Some animals received the heterotopic heart transplant and the others the kidney transplant from the transgenic pig donors to the baboons. The experiments are in progress and the data being generated from those experiments is under continuous evaluation. The initial phase of experiments will address the immunologic questions of graft rejection upon transplantation. These studies will utilize heterotopic transplants attached to an artery and vein in the neck of recipients treated with cyclosporine and leflunomide. Control animals will receive hearts from non-transgenic swine. In a separate experiment we plan to perform a small number of isolated heterotopic kidney grafts obtained from the same transgenic swine cardiac donor as a pilot for possible future research. Early results suggest that modified cardiac xenografts which

心脏切开术后心源性休克是一种重要的临床疾病 该问题将影响大约 1% 接受治疗的患者 今年心脏手术。 机械心室的经验 布莱根妇女医院为这些患者提供援助 以及全国范围内的情况非常令人失望，而且只大约 在接受传统技术支持的此类患者中，25% 将 存活到放电。 我们非常有兴趣追求 使用转基因猪异种移植物的可能性 提供临时心脏支持。 到目前为止，我们已经执行了 对11只狒狒进行移植手术。 一些动物接受了 异位心脏移植和其他肾移植 狒狒的转基因猪供体。 实验是在 这些实验的进展和数据正在研究中 持续评估。 实验的初始阶段将解决 移植排斥反应的免疫学问题。 这些研究将利用附着在 用环孢素治疗的受者颈部的动脉和静脉 和来氟米特。 对照动物将接受来自 非转基因猪。 在一个单独的实验中，我们计划执行 少量分离的异位肾移植物 相同的转基因猪心脏捐赠者作为未来可能的试点 研究。 早期结果表明改良的心脏异种移植物 哪个