VTA DA Neuron Electrophysiology in Selected Rat Lines

选定大鼠系的 VTA DA 神经元电生理学

• 批准号: 6466561
• 负责人: SANDRA L MORZORATI
• 金额: $ 11.18万
• 依托单位: INDIANA UNIV-PURDUE UNIV AT INDIANAPOLIS
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-04-01 至 2007-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6466561
• 关键词: NMDA receptors; action potentials; alcoholic beverage consumption; alcoholism /alcohol abuse; cryostat; dopamine; dopamine receptor; electrophysiology; genetic susceptibility; iontophoresis therapy; laboratory rat; mesencephalon; neurons; neuroregulation; preference; stimulant /agonist; substance abuse related behavior; substantia nigra

DESCRIPTION (provided by applicant): The long-term objective of this R01 is to identify, using electrophysiological techniques, CNS mechanisms mediating high alcohol seeking behavior. The specific aims are designed to test the hypothesis that innate differences in the mesolimbic dopamine (DA) system underlie genetic vulnerability to high alcohol seeking behavior. Rat lines selectively bred for disparate alcohol drinking will be used: the alcohol-preferring P, alcohol-non-preferring NP, high alcohol drinking HAD and low alcohol drinking LAD lines. The individual experiments will focus on the neuronal activity of ventral tegmental area (VTA) DA neurons. Extracellular recording techniques and iontophoresis will be used to determine differences in basal activity among P, NP, HAD and LAD rats, differences in receptor sensitivities to NMDA in P and NP rats and differences in neuronal activity in P and HAD rats during chronic alcohol consumption and abstinence. The results of this project will provide fundamental insights regarding electrophysiological parameters involved in high alcohol seeking behavior. Such information will be germane for developing pharmacotherapies for the treatment of alcoholism and alcohol abuse.

描述（由申请人提供）：该 R01 的长期目标是 使用电生理学技术识别介导高水平的中枢神经系统机制 寻求酒精的行为。具体目标旨在检验假设 中脑边缘多巴胺（DA）系统的先天差异是遗传的基础 容易出现酗酒行为。选择性培育的大鼠品系 将使用不同的饮酒方式：喜欢饮酒的 P， 不喜欢酒精的 NP、大量饮酒的 HAD 和少量饮酒 LAD 线。个别实验将集中于神经元活动 腹侧被盖区 (VTA) DA 神经元。细胞外记录技术和 离子电渗疗法将用于确定 P、 NP、HAD 和 LAD 大鼠，P 和 NP 中 NMDA 受体敏感性差异 大鼠以及慢性期 P 和 HAD 大鼠神经元活动的差异 饮酒和禁欲。该项目的成果将提供 关于高相关电生理参数的基本见解 寻求酒精的行为。此类信息将与开发密切相关 用于治疗酒精中毒和酒精滥用的药物疗法。