TROPOELASTIN REQUIREMENT FOR EXTRACELLULAR REPAIR OF LUNG ELASTIN FIBERS

肺弹性蛋白纤维细胞外修复的原弹性蛋白需求

• 批准号: 6478985
• 负责人: PHILLIP J STONE
• 金额: $ 5.36万
• 依托单位: BOSTON UNIVERSITY MEDICAL CAMPUS
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-07-01 至 2002-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6478985
• 关键词: biomedical resource; plants; technology /technique

Oligosaccharides that block the adherence of bacteria to epithelial cells in vitro-lacto-N-neotetraose (LNnT) and its alpha 2-1,3- and alpha 2-6-sialylated derivatives were tested for their abilities to attenuate the course of pneumococcal pneumonia and to prevent colonization of thenasopharynx in animal models. Intratracheal administration of these agents concurrently with bacteria dramatically decreased pneumococcal load in the lungs of rabbits and conferred protection from bacteremia. Several oligosaccharides were prepared by a combination of chemical andenzymatic synthesis. The samples ameliorated pneumonia and bacteremia when given therapeutically 24 h after infection was established. When administered intranasally, neoglycoconjugates of the active oligosaccharides prevented colonization of the nasopharynx of infant rats. In addition to in-vitro anti-adherence properties, LNnT acted directly on cultured lung epithelial cell lines to induce changes such that pneumococcal adherence was prevented for prolonged periods. These activities have encourage continued development of oligosaccharides as a class of potentially preventive and therapeutic agents for infectious diseases. The work with the MS Resource is to carefully check the products synthesized for sequence and linkage. Additional concern for the compounds prepared is that we have some assessment for purity.

阻断细菌遵守的寡糖 体外细胞中的上皮细胞（LNNT）及其α 测试了2-1,3-和alpha 2-6-亚苷的衍生物的衍生物 减轻肺炎球菌肺炎的能力 防止动物模型中咽咽的殖民化。 这些药物的气管内给药 细菌大大降低了肺中肺炎球菌荷荷 兔子和赋予菌血症的保护。 一些 通过化学的组合制备寡糖 雄性合成。 样品改善了肺炎和 感染后24小时治疗时细菌血症为 已确立的。 当鼻内施用时， 活性寡糖阻止了鼻咽的定殖 婴儿大鼠。 除了体外抗粘附特性外， LNNT直接作用于培养的肺上皮细胞系，以诱导 变化使肺炎球菌依从性延长了 时期。 这些活动鼓励继续发展 寡糖作为一类潜在的预防和治疗 传染病的特工。 MS资源的工作是 仔细检查合成的序列和链接的产品。 准备准备的化合物的其他问题是我们有一些 评估纯度。