IMMUNOSCANNING ELECTRON MICROSCOPY OF HERPES VIRUSES

疱疹病毒的免疫扫描电子显微镜

• 批准号: 6117285
• 负责人: Charles F. Grose
• 金额: $ 1.13万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-30 至 2000-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6117285
• 关键词: animal tissue; bioimaging /biomedical imaging; biomedical resource; infection; microscopy; vaccines; virus

The surface of the herpesvirion has not been rigorously analyzed in recent years, even though recombinant DNA technology has allowed reconstruction of synthetic capsids. The interest of this laboratory is directed towards the structure: function relationships of viral glycoproteins found in the envelope which surrounds the herpesviral capsid. In a recent study using high resolution immunoscanning electron microscopy (J.E.M. 46: 171, 1997:, we demonstrated markedly different patterns of glycoprotein localization on the surface of the virion. The above study also failed to demonstrate the typical "spike forms" which have been traditionally described in older publications. Future studies will examine the viral surface projections at a higher resolution.

疱疹病毒粒子的表面尚未经过严格分析 近年来，尽管重组 DNA 技术已经允许 合成衣壳的重建。 这个实验室的兴趣 针对病毒的结构：功能关系 在疱疹病毒周围的包膜中发现糖蛋白 衣壳。 在最近的一项使用高分辨率免疫扫描的研究中 电子显微镜 (J.E.M. 46: 171, 1997:, 我们显着地证明了 糖蛋白在表面的不同定位模式 病毒粒子。 上述研究也未能证明典型的“尖峰”现象 形式”，传统上在较旧的出版物中进行了描述。 未来的研究将在更高的水平上检查病毒表面预测 解决。