Functional human neural networks grown on 3D laser printed scaffolds

在 3D 激光打印支架上生长的功能性人类神经网络

• 批准号: 1941790
• 金额: --
• 依托单位: Aston University
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2017
• 资助国家: 英国
• 起止时间: 2017 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-1941790
• 关键词: Functional; human; neural; networks; grown

Background: The last 10 years has seen a revolution in Biology and in Photonics Engineering. Namely, the development of methods to derive specific types of cells e.g. neurons from human induced pluripotent stem cells (iPSCs), and in photonics, the development of 2 Photon Polymerisation (3D laser printing), which allows microfabrication of substates with submicron resolution. In the brain, neurons function in defined 3D networks where the specific cellular and synaptic relationships are critical for effective function. To emulate human neuronal network function in culture, it is therefore, necessary to grow them in three dimensions.Project: The aim of the project is to determine how 3D scaffold structure affects neuronal network architecture and function. This will involve 3D printing, growth of iPSC derived neurons and functional electrophysiological and calcium imaging of neuronal networks. The Student will also interact with the wider international membership of the recently awarded European FET Consortium, MESO-Brain, which comprises of Neuroscientists, Stem Cell Biologists, Physicists and Photonics experts.

背景：过去十年中，生物学和光子学工程的革命。也就是说，开发了推导特定类型细胞的方法的开发，例如来自人类诱导多能干细胞（IPSC）的神经元以及在光子学中，开发了2个光子聚合（3D激光打印），从而可以通过亚微分辨率进行微分化。在大脑中，神经元在定义的3D网络中起作用，其中特定的细胞和突触关系对于有效功能至关重要。为了模仿文化中的人类神经元网络功能，有必要在三个维度上生长它们。项目：该项目的目的是确定3D支架结构如何影响神经元网络架构和功能。这将涉及3D打印，IPSC衍生神经元的生长以及神经元网络的功能生理和钙成像。该学生还将与最近授予的欧洲FET联盟的更广泛的国际会员互动，其中包括神经科学家，干细​​胞生物学家，物理学家和光子学专家。