IMMUNE PEPTIDES IN AEDES AEGYPTI

埃及伊蚊的免疫肽

• 批准号: 6510949
• 负责人: TIMOTHY P. YOSHINO
• 金额: $ 20.97万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-04-01 至 2005-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6510949
• 关键词: Aedes; Plasmodium; Sindbis virus; Wuchereria; antigen antibody reaction; antimicroorganism antibody; blood proteins; complementary DNA; disease vectors; gene expression; genetic transcription; gram negative bacteria; immunoregulation; in situ hybridization; mass spectrometry; matrix assisted laser desorption ionization; molecular cloning; northern blottings; polymerase chain reaction; pore forming protein; proline; protein purification; protein sequence

DESCRIPTION (Adapted from the Applicant's Abstract): This proposal from a new investigator is directed towards the characterization of 4 molecules from immune-activated Ae. aegypti mosquitoes. Using MALDI-TOF MS in collaboration with P. Bulet (CNRS, Strasbourg, France), the PI has obtained evidence for at least 15 immune-inducible peptides in Ae. aegypti. Preliminary data support the analysis of a cecropin (CEC), a protein active against Gram negative bacteria (GR), a proline-rich peptide (PRO) and a 690 Da molecule (M690). Genes encoding these four molecules will be cloned, using PCR and related technologies, and the antimicrobial spectrum of the peptides will be determined. Finally, the proteins will be expressed in mosquitoes using a Sindbis vector, and the mosquitoes will subsequently be exposed to an infectious bloodmeal (Plasmodium gallinaceum and Brugia malayi) to determine the effects of the peptides, alone and in combination, on parasite development.

描述（改编自申请人的摘要）：此提案 一个新的研究者针对4的表征 免疫激活的AE分子。埃及蚊子。使用maldi-tof MS与P. Bulet（CNRS，法国Strasbourg）合作，PI具有 获得了AE中至少15种免疫诱导肽的证据。 埃及。初步数据支持Cecropin（CEC）的分析，A 蛋白质对革兰氏阴性细菌（GR）的活性，一种富含脯氨酸的蛋白 肽（Pro）和690 DA分子（M690）。编码这四个的基因 分子将使用PCR和相关技术克隆，并将 将确定肽的抗菌光谱。最后， 蛋白质将使用sindbis载体在蚊子中表达，然后 蚊子随后将暴露于感染性血液中 （加里尼亚氏疟原虫和马来语的布鲁吉亚）确定 单独和结合寄生虫发育的肽。