DEFENSIN GENE REGULATION AND MOSQUITO VECTOR COMPETENCE

防御素基因调控和蚊子载体能力

• 批准号: 6534193
• 负责人: BRUCE MARTIN CHRISTENSEN
• 金额: $ 26.65万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-01 至 2004-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6534193
• 关键词: Aedes; Culicidae; Plasmodium; Sindbis virus; Wuchereria; clinical research; defensins; disease vectors; gene expression; genetic regulation; genetic regulatory element; host organism interaction; human subject; humoral immunity; microorganism immunology; molecular cloning; parasite infection mechanism; transfection

DESCRIPTION (Adapted from the Applicant's Abstract): Defensins are the predominant immune peptide produced by Aedes aegypti in response to microbial insult, and a growing amount of data suggest this molecule, either alone or in concert with other immune factors, can interfere with the development of filarial worms and malaria parasites, if the defensin gene is induced early in an infection. To address the overall hypothesis that defensins and the antimicrobial humoral response of mosquitoes can limit the development of eukaryotic parasites, the following specific aims are proposed: (1) To identify and characterize those mechanisms regulating gene expression of defensins in Ae. Aegypti, with the long-term goal of determining how parasites are able to avoid turning on this immune response; (2) to evaluate the biology of defensins regarding their temporal and spatial expression patterns, the potential existence of a primitive immunological memory system, and their ability to kill eukaryotic parasites in vitro and in vivo; (3) to use sequence data available for immune peptides in Aedes and Anopheles mosquitoes to begin the isolation and characterization of immune peptides in Culex and to determine if immune activation limits the development of Wucheria bancrofti in this natural vector; and, (4) to use transducing viruses and transposon-mediated transformation to critically evaluate the ability of defensins and/or other immune peptides to kill filarial worms and malaria parasites in Ae. aegypti and Cx. pipiens. Studies with Cx. Pipiens and W. bancrofti are possible because of a collaborative arrangement with researchers in Egypt.

描述（根据申请人的摘要改编）：防御素是 伊德斯伊蚊产生的主要免疫肽响应微生物 侮辱和越来越多的数据表明该分子是单独或 与其他免疫因素的音乐会可以干扰 丝状蠕虫和疟疾寄生虫，如果在早期诱导防御素基因 感染。解决防御素和防御的总体假设 蚊子的抗菌体液反应可以限制 真核生物寄生虫，提出以下特定目的：（1）识别 并表征调节防御素基因表达中防御素的机制 Ae。埃及，其长期目标是确定寄生虫如何能够 避免打开这种免疫反应； （2）评估防御素的生物学 关于它们的时间和空间表达模式，潜力 原始免疫记忆系统的存在及其杀死能力 体外和体内的真核生物寄生虫； （3）使用可用的序列数据 为了使艾德斯和蚊子中的蚊子中的免疫肽开始分离 和表征Culex中免疫肽并确定是否免疫 激活限制了该天然矢量中wucheria bancrofti的发展； （4）使用转导病毒和转座子介导的转换为 批判性评估防御素和/或其他免疫肽的能力 在AE中杀死丝状蠕虫和疟疾寄生虫。埃及和CX。 pipiens。 CX的研究。 Pipiens和W. Bancrofti是可能的 与埃及研究人员的合作安排。