Sensing local biomolecular environments with coherent optical nanoscopy

使用相干光学纳米镜感测局部生物分子环境

• 批准号: 1941129
• 金额: --
• 依托单位: CARDIFF UNIVERSITY
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2017
• 资助国家: 英国
• 起止时间: 2017 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-1941129
• 关键词: Sensing; local; biomolecular; environments; coherent

The development of novel imaging tools and technologies, including super-resolution optical microscopy, has revolutionised our understanding of biological processes in living cells. Combining high resolution imaging capability with chemical sensing in living cells has the potential to unravel processes virtually impossible to follow with available techniques, such as for example defining the local environment at the specific entry site of endocytic routes of key importance for drug delivery.The main aim of this project is to demonstrate and quantify the applicability of novel optical microscopy techniques beyond state-of-the-art for local biosensing of single biomolecules directly within living cells. Specifically, the method will feature a unique combination of coherent anti-Stokes Raman scattering (CARS) [1] microscopy of endogenous biomolecules and Four-Wave Mixing (FWM) imaging of metallic nanoparticles [2], with the aim to exploit the local field enhancement effect of CARS in the vicinity of a plasmonic nanoparticle for bio-sensing at the nanoscale [3,4]. A unique home-built CARS/FWM microscope is available in Borri's laboratory [4,5], therefore the main emphasis of this project will be to push its applicability for sensing directly in living cells.[1] A Zumbusch, W Langbein, P Borri "Nonlinear vibrational microscopy applied to lipid biology" Progress in lipid research 52 (4), 615-632 (2013).[2] F. Masia, et al., Opt. Lett. 34, 1816 (2009).; ibid Phys Rev B 85, 235403 (2012).[3] H Xu et al "Electromagnetic contribution to single-molecule sensitivity in surface enhanced Raman scattering" Phys. Rev. E 62, 4318 (2000).[4] Lukas Payne, et al. "Optical micro-spectroscopy of single metallic nanoparticles: quantitative extinction and transient resonant four-wave mixing", Faraday Discussions 184, 305 (2015).[5] I Pope, et al. "Simultaneous hyperspectral differential-CARS, TPF and SHG microscopy with a single 5 fs Ti: Sa laser" Optics express 21 (6), 7096-7106 (2013).

包括超分辨率光学显微镜在内的新型成像工具和技术的开发彻底改变了我们对活细胞生物过程的理解。将高分辨率的成像能力与活细胞中的化学传感结合在一起，有可能阐明实际上无法遵循可用技术的过程，例如，例如在特定的内吞式途径的特定进入途径的局部环境中定义了对药物输送的关键重要性的特定入境地点。该项目的主要目的是该项目的主要单元格中的新型光学细胞的适用性，而不是直接的局部生物元素。该方法具体而言，该方法将具有一致的抗烟药拉曼散射（CAR）[1]内源性生物分子和四波混合（FWM）成像的独特组合[2] [3,4]。 Borri的实验室[4,5]有一个独特的家居汽车/FWM显微镜，因此该项目的主要重点是推动其直接在活细胞中感测的适用性。[1] Zumbusch，W Langbein，P Borri“应用于脂质生物学的非线性振动显微镜”脂质研究进度52（4），615-632（2013）。[2] F. Masia等人，选择。 Lett。 34，1816（2009）。同上Phys Rev B 85，235403（2012）。[3] H Xu等人“在表面增强的拉曼散射中对单分子灵敏度的电磁贡献”。 Rev. E 62，4318（2000）。[4]卢卡斯·佩恩（Lukas Payne）等。 “单金属纳米颗粒的光学微光谱：定量灭绝和瞬态谐振四波混合”，法拉第讨论184，305（2015）。[5]我教皇等人。 “与单个5 FS TI：SA Laser的同时高光谱差异卡车TPF和SHG显微镜” Optics Express 21（6），7096-7106（2013）。