A genetic screen for novel limb development genes

新型肢体发育基因的遗传筛选

• 批准号: 6594354
• 负责人: RUSSELL T COLLINS
• 金额: $ 0.55万
• 依托单位: EUROPEAN MOLECULAR BIOLOGY LABORATORY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-07-15 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6594354
• 关键词: Drosophilidae; biological signal transduction; cell growth regulation; cell proliferation; cellular polarity; developmental genetics; embryo /fetus protein; functional /structural genomics; gene expression; gene mutation; gene targeting; genetic mapping; genetically modified animals; histogenesis; immunologic techniques; in situ hybridization; invertebrate embryology; lethal genes; limbs; molecular cloning; morphology; morphometry; nucleic acid sequence; protein structure function

DESCRIPTION (provided by applicant) One of the important goals of developmental biology is to understand processes of limb morphogenesis. Remarkably, the many genes that control these processes have been conserved between insects and vertebrates, and the wing of Drosophila has proven to be a useful model for studying limb development. As a genetic tool, Drosophila is also a powerful system for the identification of genes required for many biological processes and the unbiased nature of forward genetics permits the identification of genes required for these processes that would not likely be found by any other means. Although a number of genes have been shown to be essential for the development of limbs, it is clear that many of the genes required for limb morphogenesis remain to be identified. Therefore, a systematic genetic screen to identify novel genes required for the specification, growth and patterning of Drosophila wings will be conducted. The method proposed for this screen is to generate clones of cells homozygous for newly induced mutations specifically in the developing wings of otherwise heterozygous flies, thus allowing the identification of genes required for limb development that may cause embryonic lethality in homozygous mutant animals. The genes disrupted by the mutations will be cloned and the roles of the encoded proteins in wing development will be studied. The identification of novel genes with this screen, and the subsequent characterization of their functions in wing morphogenesis, will advance our understanding of limb development.

描述（由申请人提供） 发育生物学的重要目标之一是理解过程 肢体形态发生。值得注意的是，控制这些过程的许多基因 在昆虫和脊椎动物之间保守，果蝇的翅膀 已被证明是研究肢体发育的有用模型。作为一个遗传 工具，果蝇也是一个强大的基因识别系统 许多生物过程所需的以及正向的公正性 遗传学允许识别这些过程所需的基因 不可能通过任何其他方式找到。尽管有很多基因 已被证明对于四肢的发育至关重要，很明显，许多 肢体形态发生所需的基因仍有待确定。 因此，需要进行系统的遗传筛选来识别基因所需的新基因。 将进行果蝇翅膀的规格、生长和图案化。这 为此筛选提出的方法是产生纯合的细胞克隆 新诱导的突变，特别是在其他正在发育的翅膀中 杂合果蝇，从而可以鉴定肢体所需的基因 可能导致纯合突变动物胚胎致死的发育。 被突变破坏的基因将被克隆，并且这些基因的作用 将研究翅膀发育中的编码蛋白质。鉴定 通过该筛选筛选新基因，以及随后对其进行表征 在翅膀形态发生中的功能，将增进我们对肢体的理解 发展。