Mechanism of the bacteriophage phi29 DNA packaging motor

噬菌体phi29 DNA包装马达的机制

• 批准号: 6487851
• 负责人: Yann R. Chemla
• 金额: $ 3.66万
• 依托单位: UNIVERSITY OF CALIFORNIA BERKELEY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-07-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6487851
• 关键词: adenosine triphosphate; atomic force microscopy; bacterial virus; bioenergetics; biomechanics; capsid; chemical kinetics; conformation; fluorescence microscopy; hydrolysis; intermolecular interaction; lasers; model design /development; molecular assembly /self assembly; physical model; protein structure function; thermodynamics; virus DNA; virus genetics; virus protein; virus replication

The mechanism by which a bacteriophage packages DNA into its capsid, an essential step in its replicative cycle, is poorly understood. Several models exist for the mechanical action of the molecular motor responsible for the translocation of the DNA into the viral head, none of which have been verified experimentally. Techniques such as optical tweezers, atomic force microscopy, and fluorescence microscopy are sensitive enough to probe molecular machines at the single-molecule level. The packaging motor of the bacteriophage phi29 packaging motor, this study will address the more general question of how mature converts chemical energy into mechanical action. Moreover, since the models developed for one type of phage are likely to be universally applicable to other related viruses, this investigation may lead to targets against which to develop new anti-viral drugs.

据了解，噬菌体将DNA包装到帽地的机制是复制循环中的重要步骤。存在几种模型，用于导致DNA转移到病毒头部的分子运动的机械作用，而这些模型均未通过实验验证。诸如光学镊子，原子力显微镜和荧光显微镜之类的技术足以在单分子水平上探测分子机。噬菌体PHI29包装电动机的包装电机将解决更普遍的问题，即成熟如何将化学能转化为机械作用。此外，由于为一种噬菌体开发的模型可能普遍适用于其他相关病毒，因此该研究可能导致靶标开发新的抗病毒药物。