IMAGE ANALYSIS FOR STEM

STEM 图像分析

• 批准号: 6444703
• 负责人: JOSEPH S WALL
• 金额: $ 29.31万
• 依托单位: BROOKHAVEN SCIENCE ASSOC-BROOKHAVEN LAB
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-04-01 至 2002-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6444703
• 关键词: biomedical resource; informatics; technology /technique

STEM mass measurements have been a mainstay of our program. Several years ago A. Steven developed a set of programs to compute radial density profiles from the projected mass profile measured directly in the STEM. This has been used in many projects involving cylindrical or spherical symmetry-An automatic program ("AutoMass") was developed for particle location, classification, alignment, and mass analysis with spherical or cylindrical density analysis, where appropriate, and is used extensively for analyzing STEM data. Several factors motivated this effort. Particle selection can be by mathematical rather than subjective criteria. Coarse sampling in STEM images creates problems with some programs, degrading the resolution, but can be handled easily in a custom program. Using two or more channels of data recorded multancously from the same pixel may create bookkeeping problems (e.g., large angle minus . small angle signals with appropriate thickness corrections to give hydrogen mapping). Various small nonlinearities in STEM signals can be corrected before or during processing. We have adapted these programs to run on a standard personal computer (PCMass). This cost and makes analysis available to many of our users who do not have image processing facilities. A second Pc program imports 3-D x-ray structures from the Protein Data Bank, projects these in various directions and compares these pixel by pixel with a STEM image, while viewing the differ~nce image and computing'the RMS difference. Small-angle scattering curves can be computed for comparison to neutron or x-ray data. STEM images for the past year are archived on our server and available over Internet or on optical disk. The PCMass program also provides a convenient viewer for these remote files and is provided along with image data.

STEM质量测量已成为我们计划的支柱。 几年前，A。Steven制定了一组计划来计算 来自预计质量曲线的径向密度曲线 直接在茎中。 这已用于许多涉及的项目 圆柱形或球形对称性 - 自动程序（“汽车”） 是针对粒子位置，分类，对齐和 质量分析与球形或圆柱密度分析，其中 适当，并广泛用于分析茎数据。 一些 因素激发了这一努力。 粒子选择可以是 数学而不是主观标准。 茎中的粗制采样 图像通过某些程序造成问题，降低了分辨率， 但是可以在自定义程序中轻松处理。 使用两个或更多 从同一像素录制的数据渠道可能会创建 簿记问题（例如，大角度负。小角度信号 具有适当的厚度校正以获得氢图）。 在茎信号中可以纠正各种小型非线性 或在处理过程中。 我们已经改编了这些程序来运行 标准个人计算机（PCMASS）。 这笔成本并进行分析 我们的许多没有图像处理的用户可用 设施。 第二个PC程序从 蛋白质数据库，以各种方向进行投射和比较 这些像素由像素与茎图像，同时查看不同的〜nce 图像和计算RMS差异。 小角度散射曲线 可以计算与中​​子或X射线数据进行比较。 STEM图像 在过去的一年中，在我们的服务器上存档并可以使用 互联网或光盘上。 PCMASS程序还提供了 这些远程文件的方便查看器，并提供 图像数据。