Core--Transgenic and knockout mouse

核心--转基因及基因敲除小鼠

• 批准号: 6480001
• 负责人: ANNEMARIE WALSH
• 金额: $ 26.82万
• 依托单位: ROCKEFELLER UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-07-01 至 2002-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6480001
• 关键词: animal colony; atherosclerosis; biomedical facility; blood lipoprotein metabolism; cryopreservation; gene targeting; genetically modified animals; transfection

The transgenic and knockout mouse core will support the work of all 4 projects of the proposed PPG. The core will be located at the Rockefeller University Laboratory Animal Research Center. The core will: 1) micro- inject DNA into fertilized eggs to create transgenic mouse lines; 2) electroporate DNA constructions into ES cells, clone and select homologous recombinants, perform blastocyst injection of correctly targeted ES cells, breed chimeras to demonstrate germ line transmission to demonstrate germ line transmission of gene knockout; and 3) cryopreserve liens of transgenic and knockout mice.

转基因和敲除小鼠核心将支持拟议 PPG 的所有 4 个项目的工作。核心将位于洛克菲勒大学实验动物研究中心。其核心将：1）将DNA显微注射到受精卵中以创建转基因小鼠品系； 2) 将DNA构建体电穿孔至ES细胞中，克隆并选择同源重组体，对正确靶向的ES细胞进行囊胚注射，培育嵌合体以证明种系传递，以证明基因敲除的种系传递； 3) 转基因和基因敲除小鼠的冷冻保存留置权。