CORE--CELL IMAGING FACILITY

核心——细胞成像设备

• 批准号: 6436431
• 负责人: Douglas J. Taatjes
• 金额: $ 7.89万
• 依托单位: UNIVERSITY OF VERMONT & ST AGRIC COLLEGE
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-08-01 至 2005-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6436431
• 关键词: atomic force microscopy; bioimaging /biomedical imaging; biomedical facility; confocal scanning microscopy; electron microscopy; fluorescence microscopy; light microscopy

Description: (Applicant's Description) The Cell Imaging Facility Shared Resource is designed to provide state-of-the-art and quality-assured morphologically oriented services to individual projects. The Facility is directed by Douglas Taatjes, Ph.D. The facility provides a range of sophisticated light and electron-microscopic services including scanning and transmission electron microscopy, research level transmission and fluorescence microscopy, confocal laser scanning microscopy and atomic force microscopy. The facility also provides laser scanning cytometry and laser capture microdissection services. In addition to the instrumentation available in the facility, experienced technologists provide assistance with tissue and sample preparation, data collection and analysis, image processing and experimental design. A range of services and assays are provided, but training by facility staff allow users to do much of their own data collection; in addition, the facility is fully networked so image and data analysis can be done at workstations separate from the microscopes, maximizing instrument time for data collection. The Cell Imaging Facility is an institutional core facility that is heavily subsidized by the UVM College of Medicine; oversight is provided by an advisory committee that the Cancer Center Director is a member of. Logged use by qualifying projects was over 1000 hours in 1999 but projections are that future use of the facility will increase due to recent addition of the Laser Capture Microdissector, Laser Scanning Cytometer, and a second research instrument for fluorescence microscopy that is heavily used by new VCC investigators. CCSG support is requested to offset 50 percent of chargeback costs for peer-reviewed qualifying cancer-related research of VCC members. This Shared Resource has been supported by the Cancer Center Support Grant since 1996.

描述：（申请人的描述）单元成像设施共享 资源旨在提供最先进的质量保证 针对单个项目的形态学服务。设施是 由道格拉斯·塔特（Douglas Taatjes）执导。该设施提供了一系列 精致的光线和电子微观服务，包括扫描和 透射电子显微镜，研究水平传播和荧光 显微镜，共聚焦激光扫描显微镜和原子力显微镜。 该设施还提供激光扫描细胞术和激光捕获 微分解服务。除了在 设施，经验丰富的技术人员为组织和样品提供帮助 准备，数据收集和分析，图像处理和实验 设计。提供了一系列服务和测定，但是设施培训 员工允许用户进行大量自己的数据收集；另外， 设施已完全联网，因此图像和数据分析可以在 工作站与显微镜分开，最大限度地提高了仪器的时间 数据收集。细胞成像设施是机构核心设施 这是由UVM医学院大力补贴的；监督是 由咨询委员会提供的癌症中心主任是成员 的。合格项目的记录使用时间超过1000小时，但 预测是由于最近的未来使用设施的使用将增加 添加激光捕获微动子，激光扫描细胞仪和A 荧光显微镜的第二个研究仪器，大量使用 新的VCC调查人员。请求CCSG支持以抵消50％ 同行评审的合格癌症相关研究的退款费用 成员。该共享资源得到了癌症中心支持的支持 自1996年以来。