IDENTIFICATION OF GLYCOPEPTIDE PROCESSING MECHANISMS IN PENICILLIUM

青霉中糖肽加工机制的鉴定

• 批准号: 6240222
• 负责人: Sandra Bonetti
• 金额: $ 1.37万
• 依托单位: COLORADO STATE UNIVERSITY-PUEBLO
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-07-01 至 1997-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6240222
• 关键词: Moniliales; cell membrane; endopeptidases; enzyme activity; enzyme inhibitors; glycopeptides; growth media; hydrolase; lipase; membrane proteins; nuclear magnetic resonance spectroscopy; protein biosynthesis; protein structure

This project proposes to examine the structures of low and high molecular weight mycelial glycopeptides from Penicillium charlesii and to determine whether these polymers serve as precursors and/or points of attachment or anchors for peptidophosphoglactomannans(PPGMS) or the extracellular glycohydrolases, phosphatases and proteases that are co-released with PPGMS. In order to establish the precursor/product relationships between the mycelial glycopeptides and the extracellular PPGMS, the structures of the cell wall, membrane, and extracellular PPGMS will be characterized. The structural relationships between the glycopeptides will be investigated by one and two dimensional NMR spectroscopy and GC/mass spectrometry in conjunction with amino acid analyses of peptide components. In addition, the biosynthetic relationships will be probed by using radiolabeled carbohydrate and peptide precursors an by biotinylation of cell walls, followed by isolation of the corresponding glycopeptides from the cell wall, membrane, and extracellular fractions. Establishing the anchor of these glycopeptides to the cell wall or membrane is important to the understanding of the physiological events leading to the release of PPGMS, hydrolytic enzymes, and potential allergens from the Penicillium charlesii.

该项目旨在研究低分子和高分子的结构 称重来自查尔斯青霉的菌丝体糖肽并测定 这些聚合物是否充当前体和/或连接点 或肽磷酸半乳甘露聚糖 (PPGMS) 或细胞外的锚 与糖水解酶、磷酸酶和蛋白酶共同释放的酶 PPGMS。 为了建立前体/产品之间的关系 菌丝体糖肽和胞外 PPGMS，结构 细胞壁、膜和细胞外 PPGMS 将 特点。 糖肽之间的结构关系 将通过一维和二维核磁共振波谱进行研究 GC/质谱联用肽的氨基酸分析 成分。 此外，还将探讨生物合成关系 通过使用放射性标记的碳水化合物和肽前体 细胞壁生物素化，然后分离相应的 来自细胞壁、细胞膜和细胞外部分的糖肽。 建立这些糖肽对细胞壁的锚定或 膜对于理解生理事件很重要 导致 PPGMS、水解酶和潜力的释放 来自查尔斯青霉的过敏原。