ULTRAFAST TEMPERATURE JUMP METHODS

超快温度跳跃方法

• 批准号: 6480857
• 负责人: C M PHILLIPS
• 金额: $ 15.58万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-08-01 至 2002-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6480857
• 关键词: biological products; biomedical resource; lasers; proteins

Energetic laser pulses (300 ?J- 2 mJ) are being utilized to excite absorptive dyes in solution. These dyes act as heat transducers, taking the optical energy and converting it to thermal energy. This thermal energy diffuses throughout the bulk solvent in ca. 100 ps, raising the temperature of the solvent on the order of 8-100 C. We are utilizing and exploring further optical parametric methods of generating the pulses that can be directly absorbed into the overtone bands of the solvent water (ca. 1.5-1.9 ?m). The temperature jump is being used to induce conformational changes associated with unfolding in proteins. Ultrafast infrared and visible probes have been developed to monitor the kinetics of these changes. Further modifications have been made to the system to allow observation of spectral changes on the ps to ms timescale. Other protein candidates are being considered, in particular those which undergo cold "naturation" such that we may observe changes associated with the folding processes.

充满活力的激光脉冲（300？J-2 MJ）被用来激发 溶液中的吸收染料。 这些染料充当热传感器， 采用光能并将其转换为热能。 这 热能扩散在CA中的整个散装溶剂中。 100 ps， 在8-100 C上提高溶剂的温度。我们是 利用和探索进一步的光学参数方法 产生可以直接吸收到叠音的脉冲 溶剂水的带（约1.5-1.9？m）。 温度的上升是 用于诱导与展开相关的构象变化 在蛋白质中。 超快红外线和可见探针已经 开发以监视这些变化的动力学。 更远 已经对系统进行了修改，以允许观察 PS上的频谱变为MS时间尺度。 其他蛋白质候选物 正在考虑，尤其是那些受到冷的人 “自然”使我们可以观察到与 折叠过程。