TECHNIQUE FOR SINGLE PARTICLE LOCALIZATION & TRACKING IN FLUORESCENCE MICROSCOPE

单粒子定位技术

• 批准号: 6349389
• 负责人: RUSSELL THOMPSON
• 金额: $ 1.06万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-01 至 2001-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6349389
• 关键词: bioengineering /biomedical engineering; biological products; biomedical equipment development; biomedical resource; lasers; technology /technique

We established a FCS setup that easily allows the combination of one- and two-photon excitation schemes without changing major optical components. By proper choice of the confocal detection system, such as pinhole diameters, volume elements of equal size can be realized what allows for an easy comparison of dye characteristics such as excitation cross-sections with both excitation alternatives on a single molecule level.

我们建立了一个FCS设置，该设置很容易允许 一单光量激发方案而不改变主要光学 成分。 通过正确选择共聚焦检测系统， 作为针孔直径，可以实现相等大小的体积元素 是什么允许轻松比较染料特征，例如 激发横截面具有两种激发替代方案 单分子水平。