ENDO & EXOCYTOSIS IN RBL 2H3 CELLS MEASURED W/ PATCH CLAMP CAPACITANCE TECHNIQUE

远藤

• 批准号: 6121951
• 负责人: RUSSELL THOMPSON
• 金额: $ 0.07万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-30 至 1999-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6121951
• 关键词: bioengineering /biomedical engineering; biomedical resource; education; technology /technique

NIH Biophysics Seminar A series of experiments using patch clamp capacitance recording has been initiated to further explore the secretion properties of RBL-2H3 cells. The technique makes use of the fact that the cell membrane area is proportional to capacitance, thus allowing measurements of the membrane area. Preliminary experiments have revealed both endocytic steps in capacitance as well as curious spikes possibly due to transient fusion events of exocytotic granules.

NIH生物物理学研讨会，使用斑块夹一系列实验 电容记录已启动以进一步探索 RBL-2H3细胞的分泌特性。 该技术利用 细胞膜区域与电容成正比的事实，因此 允许测量膜区域。 初步实验 已经揭示了电容和好奇的内吞步骤 尖峰可能是由于胞吐颗粒的短暂融合事件所致。