Improved cell culture systems for Hepatitis C virus

改进的丙型肝炎病毒细胞培养系统

• 批准号: 6443281
• 负责人: JAMES B HICKS
• 金额: $ 9.83万
• 依托单位: VIROGENOMICS, INC.
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-06-01 至 2003-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6443281
• 关键词: biotechnology; cell type; clinical research; hepatitis C virus; human tissue; macrophage; microorganism growth; technology /technique development; tissue /cell culture; virus RNA; virus replication

DESCRIPTION (provided by applicant): Hepatitis C virus is rapidly becoming one of the most important infectious health problems and is approaching epidemic status world wide. The CDC estimates that HCV may have already infected more that three million people in the US alone. Treatments for the chronic liver disease that eventually occurs years after HCV infection are limited to interferon and a few experimental antiviral compounds. One of the biggest obstacles to finding new drugs is the lack of a useful culture system for the virus. Dr. Jay Nelson and co-workers have discovered and cultured several new types of human cells that are apparently more permissive for viral replication. Virogenomics has been testing these cells for their ability to support infection and repliction HCV with some encouraging results. The aim of this Phase I project is confirm replication of HCV in activated macrophages or transformed hepatic endothelial cells in culture using a very sensitive TaqMan assay to verify replication by detecting production negative strand RNA. Success in the Phase I part of the project will lead to extended Phase II goals that will aim to create a robust culture system suitable fordrug screening, and look for alterations in gene expression caused by HCV infection through microarray analysis that could yield new host gene targets for blocking the pathogenic effects of the virus.

描述（由申请人提供）：乙型肝炎病毒正在迅速成为一个 最重要的传染性健康问题，正在接近流行病 地位在世界范围内。 CDC估计HCV可能已经感染了更多 仅在美国，那300万人。慢性肝脏的治疗 HCV感染后数年最终发生的疾病仅限于 干扰素和一些实验性抗病毒化合物。最大的 寻找新药的障碍是缺乏有用的文化系统 病毒。杰伊·尼尔森（Jay Nelson）博士和同事发现并培养了一些新的 显然更允许病毒复制的人类细胞类型。 病毒组学一直在测试这些细胞的支持能力 通过一些令人鼓舞的结果，感染和回答HCV。这个目的 第一阶段项目已确认在活化巨噬细胞中的HCV复制或 使用非常敏感的塔克人在培养中转化肝内皮细胞 测定通过检测产生负链RNA来验证复制的测定。 在项目的第一阶段中的成功将导致扩展II期目标 这将旨在创建适合福特鲁克筛查的强大文化系统，并且 寻找通过HCV感染引起的基因表达改变 微阵列分析可能会产生新的宿主基因靶标的来阻止 病毒的致病作用。