Diagnostic Electron Microscopy (EM)

诊断电子显微镜 (EM)

• 批准号: 6433406
• 负责人: MARIA TSOKOS
• 金额: --
• 依托单位: DIVISION OF CLINICAL SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6433406
• 关键词: Ewing's tumor; cell growth regulation; child (0-11); clinical research; clinical trial phase I; colony stimulating factor; colorectal neoplasms; dendritic cells; diagnosis design /evaluation; diagnosis quality /standard; diagnosis service; electron microscopy; health care referral /consultation; human subject; human therapy evaluation; human tissue; immunomodulators; leukocyte activation /transformation; meeting /conference /symposium; melanoma; neoplasm /cancer classification /staging; neoplasm /cancer diagnosis; neoplasm /cancer immunotherapy; neoplasm /cancer pharmacology; neoplasm /cancer remission /regression; pediatric neoplasm /cancer; prognosis; tumor necrosis factor alpha

Transmission electron microscopy (EM) is used as an adjunct procedure in the diagnosis of a wide range of neoplastic and non neoplastic diseases and can be extremely useful in well chosen diagnostic dilemmas. The main objectives of the diagnostic EM service are: (1) to provide EM diagnosis in patient specimens (2) to contribute to the understanding of the pathogenesis of diseases studied in various NIH protocols and (3) to assist investigators in research protocols with specific EM-related questions. The last year's accomplishments are summarized below:(1) A total of 122 patient tissues were processed and diagnosed by EM. Successful CAP inspection occurred on 2/15/00 leading to CAP certification (2) An additional 117 research specimens with multiple samples per specimen were processed and evaluated, resulting in 5 publications. (3) We explored the possibility to use a non radioisotopic in situ polymerase chain reaction at the ultrastrucutral level for the detection of oncogenes. An abstract submitted to the USCAP Academy of Pathology meeting in March of 1999 (Lab Invest 2000; 80:225A (1325)) won honorary mention from the Society of Ultrastructural Pathology (4) Immuno EM was introduced to assist NIH investigators in resolving questions with clinical relevance. Examples and puplications are shown below: (a) Immuno EM labeling showed the human immunodeficiency virus (HIV) envelope protein gp4 to accumulate as an extracellular aggregate in the brains of HIV-infected patients with dementia - Caffrey, M. et al: J Biol Chem 2000; 275:19877-19882 .(b) Immuno EM labeling showed that in patients susceptible to isoflurane-induced hepatitis, isufluorane metabolites bind to liver proteins and are localized in mitochondria- Njoku, D. et al: (Submitted).(c) Immuno EM labeling showed sites of leptin localization in cultured preadipocytes and mature tissue adipocytes- Bornstein, S.R. et al. Diabetes 2000; 49:532-538 .

透射电子显微镜（EM）用作诊断广泛的肿瘤和非肿瘤疾病的辅助程序，并且在选择精良的诊断困境中非常有用。诊断性EM服务的主要目标是：（1）在患者标本（2）中提供EM诊断，以有助于理解各种NIH方案中研究的疾病的发病机理，以及（3），以帮助研究人员在研究方案中使用特定的EM相关问题。下面总结了去年的成就：（1）总共处理了122个患者组织并由EM诊断。在2/15/00进行了成功的CAP检查，导致CAP认证（2）另外每个样品和评估的117个带有多个样品的研究标本，导致了5个出版物。 （3）我们探索了在超做基础水平上使用非放射性药物的原位聚合酶链反应以检测癌基因的可能性。 1999年3月提交的一份摘要（Lab Invest 2000; 80：225A（1325））赢得了超微结构病理学会（4）介绍Immuno EM的荣誉提及，以协助NIH研究人员解决具有临床相关性的问题。例子和化作如下所示：（a）免疫标记显示人类免疫缺陷病毒（HIV）包膜蛋白GP4在受HIV感染的痴呆症患者的大脑中积聚为痴呆症患者的细胞外聚集体-Caffrey -Caffrey，M。M.等人：J Biol Chem：J Biol Chem 2000; 275:19877-19882 .(b) Immuno EM labeling showed that in patients susceptible to isoflurane-induced hepatitis, isufluorane metabolites bind to liver proteins and are localized in mitochondria- Njoku, D. et al: (Submitted).(c) Immuno EM labeling showed sites of leptin localization in cultured preadipocytes and mature组织脂肪细胞 - Bornstein，S.R。等。糖尿病2000； 49：532-538。