DIAGNOSTIC ELECTRON MICROSCOPY (EM)

诊断电子显微镜 (EM)

• 批准号: 6290823
• 负责人: MARIA TSOKOS
• 金额: --
• 依托单位: DIVISION OF CLINICAL SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6290823
• 关键词: Ewing's tumor; cell growth regulation; child (0-11); clinical research; clinical trial phase I; colony stimulating factor; colorectal neoplasms; dendritic cells; diagnosis design /evaluation; diagnosis quality /standard; diagnosis service; electron microscopy; health care referral /consultation; human subject; human therapy evaluation; human tissue; immunomodulators; leukocyte activation /transformation; meeting /conference /symposium; melanoma; neoplasm /cancer classification /staging; neoplasm /cancer diagnosis; neoplasm /cancer immunotherapy; neoplasm /cancer pharmacology; neoplasm /cancer remission /regression; pediatric neoplasm /cancer; prognosis; tumor necrosis factor alpha

Transmission electron microscopy (EM) is used as an adjunct procedure in the diagnosis of a wide range of neoplastic and non neoplastic diseases and can be extremely useful in well chosen diagnostic dilemmas.The main objectives of the diagnostic EM service are: (1) to provide EM diagnosis in patient specimens (2) to contribute to the understanding of the pathogenesis of diseases studied in various NIH protocols and (3) to assist investigators in research protocols with specific EM-related questions. The last years accomplishments are summarized below. (1) A total of 118 patient tissues were processed and diagnosed by EM. (2) An additional 172 research specimens were processed and evaluated for research protocols (3) EM was instrumental in identifying the mitochondrial damage caused by the drug fialuridine that was used in NIH trials (4) EM was instrumental in defining that the achlorydric gastritis that was detected in patients with mycolipidosis type IV by NIH cllnicians was not due to lack of parietal cells, but rather to dysfunctional parietal cells because of storage material in giant lysosomes. (5) EM was important in showing that patients with the dysmorphic Smith-Lemli-Opitz syndrome, in addition to their primary defect in cholesterol biosynthesis may have a secondary defect in the degradation of intracellular low denisity lipoproteins (LDL), resulting in abnormal accumulation of unesterified sterols and lysosomal inclusions similar to those seen in Niemann Pick disease. (6) EM played a role in the morphologic characterization of reticulum cell neoplasms in lymph nodes. - Electron microscopy, - Human Tissues, Fluids, Cells, etc.

传播电子显微镜（EM）在诊断广泛的肿瘤和非肿瘤疾病的诊断中用作辅助程序，并且在选择良好的诊断困境中非常有用，诊断性EM服务的主要目标是：（1）为患者的诊断提供了对各种诊断的EM诊断（2）的研究，以促进各种研究的研究，并在研究中进行了研究。具有特定EM相关问题的协议。最后几年的成就将在下面总结。 （1）总共处理了118个患者组织并通过EM诊断。 （2）对研究方案（3）进行了另外172份研究样本，并在NIH试验（4）中使用的药物fialuridine造成的线粒体损害（4）对识别的线粒体损害有助于确定在nih cllorydnical ty ty the the Ii ii ii ii ii ii ii ii ii ii ii ii ii ii的患者中，该药物（4）在niH试验（4）中起到了作用。由于巨型溶酶体中的储存材料，功能失调的顶叶细胞。 （5）EM在表明表明患有胆固醇生物合成中的主要缺陷外，其主要缺陷可能在降解细胞内低可分离性脂蛋白（LDL）的降解中可能存在次要缺陷，因此很重要。 （6）EM在淋巴结中网状细胞肿瘤的形态表征中发挥了作用。 - 电子显微镜， - 人体组织，流体，细胞等。