Superresolution and correlative microscopy to study cell adhesion on nanopatterned substrates

超分辨率和相关显微镜研究纳米图案基底上的细胞粘附

• 批准号: 1804171
• 金额: --
• 依托单位: University of Glasgow
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2016
• 资助国家: 英国
• 起止时间: 2016 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-1804171
• 关键词: Superresolution; correlative; microscopy; study; cell

As cells adhere to surfaces, they form focal adhesions which link the cells mechanically to their surroundings but also provide important signalling too. With the invention of superresolution microscopy it has become possible to study the proteins involved in these adhesions at a molecular level. The improvement in detail is typically 10 times with a resolution of about 20 nm. At the same time semiconductor technologies have made it possible to control patterns with a precision similar to the size of the proteins. This opens up the possibilities to potentially manipulate the adhesions at a molecular level. These interactions can be visualised with a combination of superresolution and scanning electron microscopy, known as correlative microscopy. This project will use advanced microscopy and genetically engineered cells to investigate the adhesion formation on a range of nanopatterned surfaces. The aim will be to correlate the formation of the focal adhesions with the design parameters with a view to produce engineered materials for regenerative medicine.

随着细胞粘附在表面上，它们会形成局灶性粘附，从而将细胞机械地与周围环境联系起来，但也提供了重要的信号传导。借助分辨率显微镜的发明，可以在分子水平上研究参与这些粘附的蛋白质。细节的改进通常为10次，分辨率约为20 nm。同时，半导体技术使控制模式的精度类似于蛋白质的大小。这打开了可能在分子水平上操纵粘附的可能性。这些相互作用可以通过超分辨率和扫描电子显微镜（称为相关显微镜）的结合来可视化。该项目将使用晚期显微镜和基因工程细胞来研究一系列纳米图案表面上的粘附形成。目的是将焦点粘连的形成与设计参数相关联，以生产用于再生医学的工程材料。