REGULATION OF CELLULAR PROLIFERATION AND DIVERSITY IN DROSOPHILA

果蝇细胞增殖和多样性的调控

• 批准号: 6432542
• 负责人: JAMES A. KENNISON
• 金额: --
• 依托单位: EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6432542
• 关键词: Drosophilidae; cell growth regulation; cell proliferation; developmental genetics; gene induction /repression; genetic models; homeobox genes; model design /development; nucleic acid sequence; transcription factor

Genes involved in the determination and differentiation of segmental identity in Drosophila melanogaster have been identified by genetic interactions with the homeotic genes already known to be required. Many new genes required for transcriptional activation or repression of the homeotic genes have been identified. We have cloned two of the genes required for transcriptional activation and one gene required for trancriptional repression. The two genes required for transcriptional activation encode subunits of the TRAP/SMCC transcriptional coactivator complex. Maternal and zygotic expressions of both genes are required for both segmentation and segmental identity. The new homeotic transcriptional repressor encodes a zinc-finger protein conserved between Drosophila and man.We have also continued our genetic and developmental characterization of the brahma chromatin remodelling complex, which is also required for transcriptional activation of homeotic genes in Drosophila. We have identified cis-regulatory elements in two target genes and have been isolating mutations that specifically disrupt interactions of brahma with these two different target genes.

通过与已经已知所需的同源基因的遗传相互作用，已经鉴定出参与果蝇中分段身份确定和分化的基因。 已经确定了转录激活或抑制同源基因所需的许多新基因。 我们已经克隆了转录激活所需的两个基因和一个trancripriptions抑制所需的基因。 转录激活所需的两个基因编码陷阱/SMCC转录共激活因子复合物的亚基。分割和分段身份都需要这两个基因的母体和二元表达。 新型同源转录阻遏物编码果蝇和人之间保守的锌指蛋白。我们还继续我们对梵天染色质重塑复合物的遗传和发育表征，这也是果蝇中同型基因转录激活的转录激活所必需的。我们已经确定了两个靶基因中的顺式调节元件，并一直在分离突变，这些突变特异性破坏了梵天与这两个不同的靶基因的相互作用。