ORAL ETHANOL DRINKING AND CNS DOPAMINE/GABA SYSTEMS

口服乙醇饮用和中枢神经系统多巴胺/GABA 系统

• 批准号: 6410020
• 负责人: HERMAN H SAMSON
• 金额: $ 17.85万
• 依托单位: WAKE FOREST UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-01-01 至 2001-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6410020
• 关键词: NMDA receptors; alcoholic beverage consumption; behavioral /social science research tag; central nervous system; chemosensitizing agent; dopamine; ethanol; ethology; gamma aminobutyrate; laboratory rat; limbic system; microinjections; neuropsychology; oral administration; prefrontal lobe /cortex; psychological reinforcement; reinforcer; self medication; serotonin receptor; stimulus /response; tegmentum

Over the last several years we have been exploring the role of the mesolimbic dopamine system in ethanol reinforcement. Our main hypothesis has been to determine, using site specific microinjection of agonists and antagonists within this system, the effects upon ethanol self- administration in an operant paradigm. Based on these findings, we know the complex interactions between these pathways and the potential specific entry point(s) for ethanol's access to these systems remains to be determined. The work proposed over the next five years will address these issues. There are 4 specific aims. Using a new behavioral technique which we have recently developed, Aim 1 we will assess the issues of specificity of site specific microinjections for ethanol reinforcement using a multiple schedule approach. Aim 2 will examine, using the same procedures, the role of the NMDA and 5HT3 receptors within this pathway to determine if they are involved with ethanol's access to this system. In Aim 3, we will use dual site microinjections to produce multiple changes within the system, in an attempt to better understand the dynamics of this system. In Aim 4, we will use another new behavioral technique, the chain schedule, to examine the role of this system in appetitive versus consummatory components of the ethanol self- administration bout. Taken together, these studies will provide more complete information as to the function of the mesolimbic mesocortical system in the control of ethanol drinking.

在过去的几年中，我们一直在探索中唇多巴胺系统在乙醇增强中的作用。我们的主要假设是使用该系统中激动剂和拮抗剂的位点特定的显微注射来确定对操作范式中乙醇自我给药的影响。基于这些发现，我们知道这些途径与乙醇访问这些系统的潜在特定入口点之间的复杂相互作用尚待确定。未来五年提出的工作将解决这些问题。有4个具体目标。 AIM 1使用我们最近开发的新行为技术，我们将使用多个时间表方法评估特定于现场的乙醇增强的特定性问题。 AIM 2将使用相同的程序检查NMDA和5HT3受体在该途径中的作用，以确定它们是否与乙醇访问该系统有关。在AIM 3中，我们将使用双站点微型注射来在系统内部产生多个更改，以便更好地理解该系统的动态。在AIM 4中，我们将使用另一种新的行为技术链时间表来检查该系统在乙醇自我管理回合的食用和完整性组件中的作用。综上所述，这些研究将提供有关中唇质皮质系统在控制乙醇饮用的中洛林比克中皮质系统功能的更多完整信息。