ACUTE PATHOGENESIS OF HIV 2287 FOLLOWING MUCOSAL INOCULATION

粘膜接种后 HIV 2287 的急性发病机制

• 批准号: 6116320
• 负责人: DAVID M ANDERSON
• 金额: $ 8.67万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-05-01 至 2000-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6116320
• 关键词: AIDS; Mammalia; Primates; immunology; infection; inflammation; lymphatic system; model design /development; sexually transmitted diseases; virus

Little is known about the dissemination of HIV immediately following infection. This experiment is designed to examine acute pathogenesis following inoculation by intravenous, intrarectal and intravaginal inoculation. In the first part of the experiment we studied the infection after intravenous inoculation. Twenty-eight juvenile macaques were assigned to the study, one as a sham control and the remaining 27 divided into groups of three at nine time points postinoculation (PI). The time points examined were 12 hours, 1 day, 2 days, 4 days, 6 days, 10 days, 14 days, 12 days, and 28 days PI. At the time of euthanasia, tissues were collected and analyzed for the presence of virus by co-culture, PCR, immunohistochemistry and in situ hybridization. This analysis is still underway; results are still preliminary.

对于感染后艾滋病毒的立即传播知之甚少。 本实验旨在检查静脉内、直肠内和阴道内接种后的急性发病机制。 在实验的第一部分，我们研究了静脉接种后的感染。 28 只幼年猕猴被分配到该研究中，其中一只作为假对照组，其余 27 只在接种后 (PI) 的 9 个时间点分为三组。 检查的时间点为PI 12小时、1天、2天、4天、6天、10天、14天、12天和28天。 安乐死时，收集组织并通过共培养、PCR、免疫组织化学和原位杂交分析病毒的存在。 这项分析仍在进行中；结果仍处于初步阶段。