FACSCALIBUR FLOW CYTOMETER SYSTEM

FACSCALIBUR 流式细胞仪系统

• 批准号: 6053834
• 负责人: RHONA N STEIN
• 金额: $ 13.15万
• 依托单位: CTR FOR MOLECULAR MEDICINE/IMMUNOLOGY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-04-01 至 2001-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6053834
• 关键词: biomedical equipment purchase; flow cytometry

A major focus of the work at the Center for Molecular Medicine and Immunology (CMMI) is immunodiagnosis and immunotherapy of cancer, in patients and in animal models. The individual projects performed at the Center all require flow cytometry support. The Center has a Becton Dickinson FACScan, which was purchased in 1989. The instrument has received continuous heavy usage over the past decade by all investigators at the Center. During the ten years since the FACScan was purchased flow cytometry technology has advanced considerably, making the instrument available today much superior in terms of productivity and performance. In addition, our needs for flow cytometry have grown during this time period. Thus, we are submitting this application for the acquisition of the FACSCaliber Flow Cytometer System with the FACSLoader, an updated flow cytometry system, which will have greater reliability, efficiency, and broader scientific applications. Specific uses of flow cytometry at the Center include: lymphocyte phenotyping, screening cell lines for reactivity with MAbs, selection of MAbs based on reactivity or lack of reactivity with tumor cell lines and/or normal cells, DNA cell cycle analysis, apoptosis studies, detection of tumor cells in stem cell preparations, analyses of dendritic cells, and absolute cell counting for toxicity evaluations in therapy studies. Administrative/scientific oversight for the instrument, including day-to- day management and technical training for in-house staff will be the responsibility of Dr. Stein, under advisement of the Center's Basic Research Committee. We estimate that the major users will require approximately 75-80% of the total usage of the instrument. In addition, the instrument will be available to less frequent users within CMMI as well as users outside of CMMI, including members of the Biological Science Department at Rutgers Newark which is located a short distance from CMMI.

分子医学和免疫学中心 (CMMI) 的主要工作重点是患者和动物模型中的癌症免疫诊断和免疫治疗。该中心进行的各个项目都需要流式细胞术的支持。该中心拥有一台 Becton Dickinson FACScan，于 1989 年购买。该仪器在过去十年中受到该中心所有研究人员的持续大量使用。自购买 FACScan 以来的十年间，流式细胞术技术取得了长足的进步，使得当今的仪器在生产力和性能方面更加优越。此外，我们对流式细胞术的需求在此期间有所增长。因此，我们提交此申请，购买带有 FACSLoader 的 FACSCaliber 流式细胞仪系统，这是一种更新的流式细胞术系统，它将具有更高的可靠性、效率和更广泛的科学应用。该中心流式细胞术的具体用途包括：淋巴细胞表型分析、筛选细胞系与 MAb 的反应性、根据与肿瘤细胞系和/或正常细胞的反应性或缺乏反应性选择 MAb、DNA 细胞周期分析、细胞凋亡研究、检测干细胞制剂中肿瘤细胞的分析、树突状细胞分析以及治疗研究中毒性评估的绝对细胞计数。该仪器的行政/科学监督，包括内部工作人员的日常管理和技术培训，将由斯坦博士负责，并在中心基础研究委员会的建议下进行。我们估计主要用户将需要仪器总使用量的大约 75-80%。此外，该工具还可供 CMMI 内不太频繁的用户以及 CMMI 之外的用户使用，包括距离 CMMI 不远的罗格斯纽瓦克大学生物科学系的成员。