SHARED LC/QUADRUPOLE ION TRAP MASS SPECTROMETER SYSTEM

共享 LC/四极杆离子阱质谱仪系统

• 批准号: 6052318
• 负责人: EDWARD A DRATZ
• 金额: $ 25.31万
• 依托单位: MONTANA STATE UNIVERSITY - BOZEMAN
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-04-01 至 2001-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6052318
• 关键词: biomedical equipment purchase; electrospray ionization mass spectrometry; high performance liquid chromatography; liquid chromatography mass spectrometry

Nine user groups are requesting funding for an LC Quadrapole Ion Trap Mass Spectrometer and accessories. The LC/MS, MS/MS, and software capabilities of the instrument requested are needed for separation and sequencing of peptides and protein digests, for characterization of bioactive natural products, and to assist in the development of natural product syntheses. The method currently in use at MSU for protein digest analysis requires repeated separate steps of protein digestion, HPLC separation, fraction collection, and finally MALDI TOF analysis of molecular weight. This current method is incapable of yielding reliable sequence information at amino acid resolution, is extremely time consuming, and is hampered by poor recovery because of the large number of steps required. Montana State University has no LC/MS, or MS/MS capabilities. The addition of an LC/Quadrapole Ion Trap Mass Spectrometer to our mass spectrometry facility will make many new experiments possible, will greatly increase the structural information content of the research, as well as enhance the effectiveness of research training at Montana State University, as described in the proposal.

九个用户团体正在申请资助购买 LC 四极离子阱质谱仪和配件。肽和蛋白质消化物的分离和测序、生物活性天然产物的表征以及协助天然产物合成的开发需要所需仪器的 LC/MS、MS/MS 和软件功能。 MSU 目前使用的蛋白质消化分析方法需要重复单独的步骤：蛋白质消化、HPLC 分离、级分收集，最后是 MALDI TOF 分子量分析。目前的方法无法在氨基酸分辨率下产生可靠的序列信息，极其耗时，并且由于需要大量步骤而受到回收率差的阻碍。蒙大拿州立大学没有 LC/MS 或 MS/MS 功能。如所述，在我们的质谱设施中添加 LC/四极离子阱质谱仪将使许多新实验成为可能，将大大增加研究的结构信息内容，并提高蒙大拿州立大学研究培训的有效性在提案中。