TSQ Quantum ESI-MS Surveyor Bundle

TSQ Quantum ESI-MS 测量仪套件

• 批准号: 6732558
• 负责人: DAVID A. FORD
• 金额: $ 33.9万
• 依托单位: SAINT LOUIS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-01 至 2005-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6732558
• 关键词: biomedical equipment purchase; electrospray ionization mass spectrometry; functional /structural genomics; high performance liquid chromatography; metabolomics; proteomics

DESCRIPTION (provided by applicant): St. Louis University has a critical mass of NIH funded investigators whose research would be enhanced substantially by the purchase of a TSQ Quantum triple quadrupole ESI-MS/Surveyor HPLC instrument bundle. To support the ongoing studies integrating genomics, proteomics and metabolomics at Saint Louis University we request funds to purchase this instrument from Thermo Finnigan. The proposed users have appointments in five different departments at the Health Sciences Center, and are involved in basic and applied research in the fields of gene regulation, lysosomal storage diseases, heart disease, renal disease, diabetes, retinal disease, pulmonary disease, drug abuse and pathogenic mycology. The rapid progress in genomics and proteomics has created new opportunities for understanding metabolic processes controlled by macromolecular complexes. Understanding these metabolic processes by identifying the diverse metabolites Produced under different physiological and pathophysiological conditions represents an important new field of biomedical research described as metabolomics. Research in this field should provide both important diagnostic strategies as well as therapeutic targets for the treatment of many diseases. Triple quadrupole electrospray ionization-mass spectrometry (ESI-MS) instrumentation with an HPLC interface is required for many metabolomic studies. The triple quadrupole is required for identification of metabolites since it can be used for collisionally induced dissociation (CID) of molecular ions which provides structurally-informative spectra. Additionally, HPLC resolved metabolites infused into the ESI source provides both important chromatographic characterization of metabolites and ESI-MS analysis of metabolites which together are often critical for the identification of metabolites. A major deficit of the instrument facility at Saint Louis University Health Sciences Center is the lack of a triple quadrupole ESI-MS instrument. This requested instrumentation will remedy this deficit in our otherwise well-equipped facility.

描述（由申请人提供）：圣路易斯大学拥有大量 NIH 资助的研究人员，通过购买 TSQ Quantum 三重四极杆 ESI-MS/Surveyor HPLC 仪器套装，可以大大增强他们的研究能力。为了支持圣路易斯大学正在进行的整合基因组学、蛋白质组学和代谢组学的研究，我们请求资金从 Thermo Finnigan 购买该仪器。提议的用户在健康科学中心的五个不同部门任职，参与基因调控、溶酶体贮积病、心脏病、肾脏疾病、糖尿病、视网膜疾病、肺部疾病、药物滥用等领域的基础和应用研究和病原真菌学。基因组学和蛋白质组学的快速进展为理解大分子复合物控制的代谢过程创造了新的机会。通过识别在不同生理和病理生理条件下产生的多种代谢物来理解这些代谢过程代表了生物医学研究的一个重要新领域，即代谢组学。该领域的研究应该为许多疾病的治疗提供重要的诊断策略和治疗靶点。许多代谢组学研究需要配备 HPLC 接口的三重四极杆电喷雾电离质谱 (ESI-MS) 仪器。三重四极杆是代谢物鉴定所必需的，因为它可用于分子离子的碰撞诱导解离 (CID)，从而提供结构信息光谱。此外，注入 ESI 源中的 HPLC 解析的代谢物可提供重要的代谢物色谱表征和代谢物的 ESI-MS 分析，这两者对于代谢物的鉴定通常至关重要。圣路易斯大学健康科学中心仪器设施的一个主要缺陷是缺乏三重四极杆 ESI-MS 仪器。所要求的仪器将弥补我们装备精良的设施中的这一缺陷。