Trafficking of proteins to the tonoplast and the effects of abiotic stress

蛋白质向液泡膜的运输和非生物胁迫的影响

• 批准号: 1782597
• 金额: --
• 依托单位: University of Warwick
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2016
• 资助国家: 英国
• 起止时间: 2016 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-1782597
• 关键词: Trafficking; proteins; tonoplast; effects; abiotic

Plants feed the world, both directly in the food that we eat and indirectly in providing feed for livestock. The world's population is predicted to increase to approximately 8 billion by 2024, and this increase, in combination with the effects of climate change, will greatly affect global agricultural production. Since a major proportion of the developing world's crops are grown in areas affected by drought and poor soil conditions research into this area is paramount in order to develop crops able to withstand such adverse conditions. Alongside being resilient, the challenge is to ensure there are no detrimental effects on crop yield or quality. Plants as sessile organisms have to constantly adapt to the changing conditions of their immediate environment. Most plant proteins essential for human and/or livestock nutrition are synthesised and stored in the plant secretory pathway. This is a system of dynamic, membrane-bound organelles comprising of the endoplasmic reticulum (ER), Golgi complex, endosomes and vacuoles. Vacuoles are the intracellular endpoint of the secretory pathway. In many seed crops, proteins are stored in special vacuoles called protein storage vacuoles (PSVs). The vacuolar membrane, the tonoplast, contains proteins such as aquaporins that act as channels for the transport of water and small molecules.To date, no definitive mechanism by which tonoplast associated proteins are trafficked to this vacuolar membrane has been described. This project aims to elucidate the sorting of membrane proteins along the secretory pathway to the tonoplast, and how this trafficking is altered under abiotic stress conditions. Results generated from this fundamental research in the model plant Arabidopsis thaliana could subsequently be translated into sustainable crop species in order to enable the development of abiotic stress tolerant crops.

植物直接在我们吃的食物和间接提供牲畜饲料时喂养世界。预计到2024年，世界人口将增加到约80亿，与气候变化的影响相结合，这将极大地影响全球农业生产。由于大部分的发展中国家农作物在受干旱影响的地区种植，因此对该地区的土壤条件不良研究至关重要，以发展能够承受这种不利条件的农作物。除了具有弹性外，挑战是确保对作物产量或质量没有不利影响。植物作为无柄生物必须不断适应其直接环境的变化条件。大多数对人类和/或牲畜营养必不可少的植物蛋白是合成并存储在植物分泌途径中的。这是一个由内质网（ER），高尔基体配合物，内体和液泡组成的动态，结合膜结合的细胞器系统。液泡是分泌途径的细胞内终点。在许多种子作物中，蛋白质存储在称为蛋白质储存液泡（PSV）的特殊液泡中。液泡膜（块状体）含有蛋白质，例如作为水和小分子运输的通道的蛋白质。到目前为止，还没有描述过将TONOPLAST相关蛋白运送到该液泡膜的确定机制。该项目的目的是阐明沿着分泌型吨位的分泌途径的膜蛋白的分类，以及在非生物应力条件下如何改变这种贩运。该模型植物拟南芥中的这项基本研究产生的结果随后可以转化为可持续的作物物种，以便能够发展非生物胁迫耐受作物。