**SSA**Characterisation of type III effector proteins from A/E pathogens.

**SSA** 来自 A/E 病原体的 III 型效应蛋白的表征。

• 批准号: 1655054
• 金额: --
• 依托单位: Imperial College London
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2015
• 资助国家: 英国
• 起止时间: 2015 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-1655054
• 关键词: SSA; Characterisation; type; III; effector

Human infections caused by Escherichia coli present a significant burden to public health. For example, several recent outbreaks can be traced back to contaminated produce that had been in contact with cattle, the natural reservoir for some pathogenic E. coli strains. These bacteria produce molecular agents that allow them to target and manipulate gut tissue in humans, leading to pathology. One group of these agents are toxins, or effector proteins, that are injected into gut cells by the bacterium via a macromolecular complex termed the type III secretion system (T3SS). This project will focus on uncharacterised effector proteins produced by pathogenic E. coli strains, as well as Citrobacter rodentium, which is a mouse-restricted pathogen that shares a number of virulence determinants with human pathogenic E. coli strains. We aim to determine the specific host pathways that are being targeted by the bacterium, the molecular basis for these interactions, and demonstrate the importance of effectors in a mouse model of infection. Understanding the role of each of these toxins in infection is critical for developing novel therapeutic agents and effectively dealing with outbreaks.

大肠杆菌引起的人类感染给公共卫生带来了重大负担。例如，最近的几次暴发可以追溯到与牛接触的受污染农产品，这是一些致病性大肠杆菌菌株的天然储层。这些细菌会产生分子剂，使它们能够靶向并操纵人类的肠道组织，从而导致病理学。这些药物的一组是毒素或效应蛋白，它们通过细菌通过大分子分子复合物将其注入肠道细胞，称为III型分泌系统（T3SS）。该项目将集中于致病性大肠杆菌菌株产生的未表征的效应蛋白，以及柠檬酸杆菌啮齿动物，这是一种小鼠限制的病原体，具有许多具有人类病原性大肠杆菌菌株的毒力决定因素。我们旨在确定细菌靶向的特定宿主途径，这些相互作用的分子基础，并证明了效应子在小鼠感染模型中的重要性。了解这些毒素在感染中的作用对于发展新型治疗剂并有效处理暴发至关重要。

项目成果

Author Correction: Host-associated niche metabolism controls enteric infection through fine-tuning the regulation of type 3 secretion.

• DOI: 10.1038/s41467-018-07612-0
• 发表时间: 2018-11-29
• 期刊: Nature communications
• 影响因子: 16.6
• 作者: Connolly JPR;Slater SL;O'Boyle N;Goldstone RJ;Crepin VF;Ruano-Gallego D;Herzyk P;Smith DGE;Douce GR;Frankel G;Roe AJ
• 通讯作者: Roe AJ