DEVELOPMENT OF FUNCTION IN THE VERTEBRATE RETINA
脊椎动物视网膜的功能发育
基本信息
- 批准号:6502779
- 负责人:
- 金额:$ 8.3万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1997
- 资助国家:美国
- 起止时间:1997-08-01 至 2002-07-31
- 项目状态:已结题
- 来源:
- 关键词:acetylcholine alternatives to animals in research calcium indicator calcium ion dendrites developmental neurobiology electrodes fluorescence microscopy glutamate receptor glutamates image processing ion transport membrane channels neurogenesis neurotransmitter antagonist neurotransmitter receptor organ culture photomicrography retinal bipolar neuron single cell analysis synapses tissue /cell preparation trout /salmon voltage /patch clamp voltage gated channel
项目摘要
Retinal development is controlled by both genetic lineage and
extracellular factors. The identity and mechanisms of action of
these factors is generally unknown, but recent experimental results
suggest that neurotransmitter molecules may play a regulatory role
in the the maturation of retinal neurons. The objective of the
research proposed here is to determine the role that
neurotransmitter molecules or their ion channel receptors play in
the maturation of ganglion cells. This objective will be pursued in
electrophysiologicla and anatomical studies of the retina in
rainbow trout. Teleost fish are unique among vertebrates because
their eyes continue to grow throughout the life of the animal.
New retinal differentiation occurs continually at the margin of the
mature retinal. This ring of newly developing retina is the
peripheral germinal zone (PGZ). In the PGX there is a gradient
of development that extends continuously from undifferentiated
stem cells at the margin to fully mature cells more centrally.
Thus, in the PGZ at any given moment, age and distance are
equivalent and all stages of retinal differentiation and maturation
exist side by side. This offers experimental advantages unmet in
mammalian systems.
The specific aims of the research plan are: To determine that
changes in the structure of the dendritic arbor of ganglion cells
associated with their developmental maturation in the PGZ. To
determine the role of glutamate and acetylcholine as regulators of
these changes. In particular, to establish whether regulation is
dependent on the presynaptic release of the neurotransmitters or
the development of postsynaptic ion channel receptors. To
determine whether these postsynaptic receptor undergo functional
changes associated with the maturation of ganglion cells and to
explore the role that these functional changes may have in
controlling developmental events. Finally, to establish whether
the neurotransmitters or their receptors are the cause of changes
associated with maturation by investigating the consequence to
retinal development of interfering with the normal changes in the
neurotransmitter systems.
视网膜发育受遗传谱系和
细胞外因素。 的身份和作用机制
这些因素通常是未知的,但最近的实验结果
表明神经递质分子可能发挥调节作用
视网膜神经元的成熟。 该计划的目标
这里提出的研究是确定的作用
神经递质分子或其离子通道受体在
神经节细胞的成熟。 这一目标将在
视网膜的电生理学和解剖学研究
虹鳟鱼。 硬骨鱼在脊椎动物中是独一无二的,因为
它们的眼睛在动物的一生中不断生长。
新的视网膜分化不断发生在视网膜边缘
成熟的视网膜。 这个新发育的视网膜环是
外周生发区(PGZ)。 在PGX中有一个梯度
的发展是从无差别不断延伸出来的
边缘的干细胞到更中心的完全成熟细胞。
因此,在任何给定时刻的 PGZ 中,年龄和距离为
视网膜分化和成熟的等效阶段和所有阶段
并存。 这提供了在以下方面未达到的实验优势:
哺乳动物系统。
研究计划的具体目标是: 确定
神经节细胞树突乔木结构的变化
与 PGZ 的发育成熟有关。 到
确定谷氨酸和乙酰胆碱作为调节剂的作用
这些变化。 特别是要确定监管是否
依赖于神经递质的突触前释放或
突触后离子通道受体的发育。 到
确定这些突触后受体是否具有功能
与神经节细胞成熟相关的变化
探索这些功能变化可能发挥的作用
控制发育事件。 最后,确定是否
神经递质或其受体是变化的原因
通过调查结果来与成熟相关
干扰视网膜发育的正常变化
神经递质系统。
项目成果
期刊论文数量(0)
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JUAN IGAL KORENBROT其他文献
JUAN IGAL KORENBROT的其他文献
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{{ truncateString('JUAN IGAL KORENBROT', 18)}}的其他基金
CELLULAR MECHANISMS OF VERTEBRATE PHOTORECEPTOR RENEWAL
脊椎动物光感受器更新的细胞机制
- 批准号:
3265595 - 财政年份:1989
- 资助金额:
$ 8.3万 - 项目类别:
CELLULAR MECHANISMS OF VERTEBRATE PHOTORECEPTOR RENEWAL
脊椎动物光感受器更新的细胞机制
- 批准号:
3265594 - 财政年份:1989
- 资助金额:
$ 8.3万 - 项目类别:
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