DEVELOPMENT OF FUNCTION IN THE VERTEBRATE RETINA

脊椎动物视网膜的功能发育

• 批准号: 2711166
• 负责人: JUAN IGAL KORENBROT
• 金额: $ 24.56万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-08-01 至 2001-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2711166
• 关键词: acetylcholine; alternatives to animals in research; calcium indicator; calcium ion; dendrites; developmental neurobiology; electrodes; fluorescence microscopy; glutamate receptor; glutamates; image processing; ion transport; membrane channels; neurogenesis; neurotransmitter antagonist; neurotransmitter receptor; organ culture; photomicrography; retinal bipolar neuron; single cell analysis; synapses; tissue /cell preparation; trout /salmon; voltage /patch clamp; voltage gated channel

Retinal development is controlled by both genetic lineage and extracellular factors. The identity and mechanisms of action of these factors is generally unknown, but recent experimental results suggest that neurotransmitter molecules may play a regulatory role in the the maturation of retinal neurons. The objective of the research proposed here is to determine the role that neurotransmitter molecules or their ion channel receptors play in the maturation of ganglion cells. This objective will be pursued in electrophysiologicla and anatomical studies of the retina in rainbow trout. Teleost fish are unique among vertebrates because their eyes continue to grow throughout the life of the animal. New retinal differentiation occurs continually at the margin of the mature retinal. This ring of newly developing retina is the peripheral germinal zone (PGZ). In the PGX there is a gradient of development that extends continuously from undifferentiated stem cells at the margin to fully mature cells more centrally. Thus, in the PGZ at any given moment, age and distance are equivalent and all stages of retinal differentiation and maturation exist side by side. This offers experimental advantages unmet in mammalian systems. The specific aims of the research plan are: To determine that changes in the structure of the dendritic arbor of ganglion cells associated with their developmental maturation in the PGZ. To determine the role of glutamate and acetylcholine as regulators of these changes. In particular, to establish whether regulation is dependent on the presynaptic release of the neurotransmitters or the development of postsynaptic ion channel receptors. To determine whether these postsynaptic receptor undergo functional changes associated with the maturation of ganglion cells and to explore the role that these functional changes may have in controlling developmental events. Finally, to establish whether the neurotransmitters or their receptors are the cause of changes associated with maturation by investigating the consequence to retinal development of interfering with the normal changes in the neurotransmitter systems.

视网膜发育均由遗传谱系和 细胞外因子。 行动的身份和机制 这些因素通常是未知的，但是最近的实验结果 表明神经递质分子可能起调节作用 在视网膜神经元的成熟中。 目的 这里提出的研究是为了确定 神经递质分子或其离子通道受体在 神经节细胞的成熟。 这个目标将被追求 视网膜的电生理学和解剖学研究 彩虹鳟鱼。 静脉鱼在脊椎动物中是独一无二的，因为 他们的眼睛在动物的整个生命中继续增长。 新的视网膜分化不断发生在 成熟的视网膜。 这圈新开发的视网膜是 外围生发区（PGZ）。 在PGX中有一个梯度 从未分化的发展的发展 干细胞在边缘更加集中到完全成熟的细胞。 因此，在任何给定时刻的PGZ中，年龄和距离为 视网膜分化和成熟的等效和所有阶段 并排存在。 这提供了实验优势未得到的 哺乳动物系统。 研究计划的具体目的是：确定 神经节细胞树突状乔木的结构的变化 与它们在PGZ中的发展成熟相关。 到 确定谷氨酸和乙酰胆碱作为调节剂的作用 这些变化。 特别是确定法规是否是 取决于神经递质的突触前释放或 突触后离子通道受体的发展。 到 确定这些突触后受体是否具有功能 与神经节细胞的成熟相关的变化和 探索这些功能变化可能在 控制发展事件。 最后，确定是否 神经递质或其受体是变化的原因 通过调查结果与成熟相关 干扰正常变化的视网膜发展 神经递质系统。