IMS/MS DETECTION OF ILLICIT DRUGS IN BIOLOGICAL SAMPLES

IMS/MS 检测生物样品中的非法药物

• 批准号: 6378461
• 负责人: LAURA E MARKOWSKI-MATZ
• 金额: $ 1.61万
• 依托单位: WASHINGTON STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-03-09 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6378461
• 关键词: amphetamines; analytical method; benzodiazepines; biological products; clinical biomedical equipment; cocaine; diagnosis quality /standard; drug detection; electrospray ionization mass spectrometry; hair; matrix assisted laser desorption ionization; opiate alkaloid; physical separation; plasma; serum; substance abuse related disorder; technology /technique development; tissues; urine

DESCRIPTION This application is submitted for predoctoral fellowship support to provide training in the analytical chemistry and biochemistry of drug analysis. It is proposed that three novel ionization techniques; electrospray ionization (ESI), secondary electrospray ionization (SESI), and matrix-assisted laser desorption ionization (MALDI), be employed with ion mobility spectrometry/mass spectrometry (IMS/MS) for drug detection. IMS is already accepted as a sensitive and selective drug detection system, although it has been limited to field monitoring applications. The three ionization techniques are expected to be less sensitive to biological matrix effects which will extend the range of drug applications for IMS/MS detection. The proposed project will; (1) evaluate each ionization techniques with IMS/MS for several classes of drugs (benzodiazepines, amphetamines, opiates, and cocaine), (2) develop procedures for each biological fluid (e.g. direct injection, pre-extraction step) and (3) compare the three ionization techniques for analytical figures of merit (sensitivity, detection limit, selectivity in each biological sample, and reproducibility). Biological samples to be tested include tissue, plasma/serum, hair, and urine. NIST illicit drug-containing standards in each biological sample type will be obtained and accuracy tests will be performed. Upon completion of these goals, further research will extend into detection of other drugs (antibiotics) and conformation/protein binding studies.

描述此申请是为了提供分析化学和药物分析的生物化学培训的培训。有人提出三种新型电离技术。电喷雾电离（ESI），二次电喷雾电离（SESI）和基质辅助激光解吸电离（MALDI），用于用于药物检测的离子迁移率谱法/质谱法（IMS/MS）。 IMS已经被认为是一种敏感和选择性的药物检测系统，尽管它仅限于现场监测应用。预计三种电离技术对生物基质效应的敏感性较小，这将扩大IMS/MS检测的药物应用范围。拟议的项目将； （1）使用IMS/MS评估每种电离技术，以用于几类药物（苯二氮卓类药物，苯丙胺，阿片类和可卡因），（2）为每种生物学流体（例如直接注射，预注射，预牵引前的步骤）和（3）比较分析限制的每个生物学（例如直接注射，预授予的限制）（比较分析效率（3）的分析效率（3）可重复性）。要测试的生物样品包括组织，血浆/血清，头发和尿液。将获得每种生物样品类型中的非法非法药物标准标准，并将进行准确性测试。完成这些目标后，进一步的研究将扩展到对其他药物（抗生素）和构象/蛋白质结合研究的检测。