A FUNCTIONAL GENOMICS HEART & LUNG DEVELOP. PROGRAM
功能基因组心脏
基本信息
- 批准号:6391254
- 负责人:
- 金额:$ 93.21万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2000
- 资助国家:美国
- 起止时间:2000-09-30 至 2004-07-31
- 项目状态:已结题
- 来源:
- 关键词:asthma biomedical resource cardiopulmonary disease chronic obstructive pulmonary disease clinical research cooperative study cystic fibrosis disease /disorder etiology functional /structural genomics gel electrophoresis gene expression genetic library genetic mapping genetic polymorphism heart failure human genetic material tag human tissue lung injury mass spectrometry matrix assisted laser desorption ionization microarray technology myocardial ischemia /hypoxia polymerase chain reaction protein structure pulmonary fibrosis /granuloma pulmonary hypertension
项目摘要
Drs. Eric Hoffman and Dietrich Stephan (whose primary interests are focused on muscle disease and leukemia) are PI and Co-PI on the Program's Expression Array Core and have extensive experience with all aspects of array use and data analysis. They have established collaborations with Dr. Michael Bitmer, Dr. Yidony Chen and the entire NHGRI array community. As a post-doctoral fellow in the laboratory of Dr. Jeffery Trent (member of this Program's External Advisory Committee), and a pioneer in DNA microarray technology, Dr. Stephan developed several alternative approaches to label total RNA for efficient signal detection under varying circumstances which have become the standard protocol used at NHGRI and has become a quite robust and reliable system for detecting signals over several orders of magnitude. In addition to building and using 7K expression arrays, Dr. Stephan was the first at NHGRI to develop genomic DNA microarray systems. Dr. Hoffman's laboratory is particularly interested in systematic assessments of the sensitivity and specificity of the Affymetrix vs. cDNA array approaches, and shows preliminary data pointing out the importance of redundant measurements, and correlative studies. Indeed, the proposed systematic comparison of the Affymetrix and cDNA array experimental platforms should be the first of this type of quality control of expression array data, and should prove highly valuable to both Program investigations and the research community at large.
博士。 Eric Hoffman 和 Dietrich Stephan(主要兴趣集中在肌肉疾病和白血病)是该计划表达阵列核心的 PI 和 Co-PI,在阵列使用和数据分析的各个方面拥有丰富的经验。他们与 Michael Bitmer 博士、Yidony Chen 博士以及整个 NHGRI 阵列社区建立了合作。作为 Jeffery Trent 博士(该计划外部咨询委员会成员)实验室的博士后研究员,以及 DNA 微阵列技术的先驱,Stephan 博士开发了几种标记总 RNA 的替代方法,以便在不同情况下进行有效的信号检测它已成为 NHGRI 使用的标准协议,并且已成为一个非常强大且可靠的系统,用于检测几个数量级的信号。除了构建和使用 7K 表达阵列之外,Stephan 博士还是 NHGRI 第一位开发基因组 DNA 微阵列系统的人。 Hoffman 博士的实验室对 Affymetrix 与 cDNA 阵列方法的敏感性和特异性的系统评估特别感兴趣,并显示初步数据,指出冗余测量和相关研究的重要性。事实上,所提出的 Affymetrix 和 cDNA 阵列实验平台的系统比较应该是这种类型的表达阵列数据质量控制的第一个,并且应该证明对计划调查和整个研究界都非常有价值。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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ERIC P. HOFFMAN其他文献
ERIC P. HOFFMAN的其他文献
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{{ truncateString('ERIC P. HOFFMAN', 18)}}的其他基金
Clinical Trial Readiness for Monitoring Muscle Inflammation in Duchenne Muscular Dystrophy
监测杜氏肌营养不良症肌肉炎症的临床试验准备
- 批准号:
10725465 - 财政年份:2023
- 资助金额:
$ 93.21万 - 项目类别:
Commercialization Readiness Pilot (CRP) to maximize vamorolone international labeling and sales
商业化准备试点 (CRP),以最大限度地提高瓦莫洛龙的国际标签和销售
- 批准号:
10200153 - 财政年份:2016
- 资助金额:
$ 93.21万 - 项目类别:
K12 Career Development Program: Omics of Pediatric Lung Diseases in DC
K12 职业发展计划:华盛顿特区小儿肺部疾病组学
- 批准号:
8857246 - 财政年份:2013
- 资助金额:
$ 93.21万 - 项目类别:
K12 Career Development Program: Omics of Pediatric Lung Diseases in DC
K12 职业发展计划:华盛顿特区小儿肺部疾病组学
- 批准号:
8575197 - 财政年份:2013
- 资助金额:
$ 93.21万 - 项目类别:
K12 Career Development Program: Omics of Pediatric Lung Diseases in DC
K12 职业发展计划:华盛顿特区小儿肺部疾病组学
- 批准号:
8722615 - 财政年份:2013
- 资助金额:
$ 93.21万 - 项目类别:
Center for Research Translation of Systemic Exon-skipping in Muscular Dystrophy
肌营养不良症系统性外显子跳跃研究转化中心
- 批准号:
8544772 - 财政年份:2011
- 资助金额:
$ 93.21万 - 项目类别:
Center for Research Translation of Systemic Exon-skipping in Muscular Dystrophy
肌营养不良症系统性外显子跳跃研究转化中心
- 批准号:
8330812 - 财政年份:2011
- 资助金额:
$ 93.21万 - 项目类别:
Center for Research Translation of Systemic Exon-skipping in Muscular Dystrophy
肌营养不良症系统性外显子跳跃研究转化中心
- 批准号:
8734214 - 财政年份:2011
- 资助金额:
$ 93.21万 - 项目类别:
Center for Research Translation of Systemic Exon-skipping in Muscular Dystrophy
肌营养不良症系统性外显子跳跃研究转化中心
- 批准号:
8090706 - 财政年份:2011
- 资助金额:
$ 93.21万 - 项目类别:
AN EXERCISE INTERVENTION IN INSULIN-RESISTANT MINORITY ADOLESCENTS
对胰岛素抵抗的少数青少年的运动干预
- 批准号:
8167332 - 财政年份:2010
- 资助金额:
$ 93.21万 - 项目类别:
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APPLIED GENOMICS IN CARDIOPULMONARY DISEASE--EDUCATION
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- 批准号:
6291596 - 财政年份:2000
- 资助金额:
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