BIOSENSOR FOR DIAGNOSIS OF PERIODONTAL DISEASES

用于诊断牙周疾病的生物传感器

• 批准号: 6379960
• 负责人: Bill W Colston
• 金额: $ 3.81万
• 依托单位: UNIVERSITY OF CALIF-LAWRNC LVRMR NAT LAB
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-08-01 至 2002-12-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6379960
• 关键词: biomedical equipment development; biosensor device; collagenase; dental disorder diagnosis; diagnosis design /evaluation; enzyme activity; enzyme linked immunosorbent assay; enzyme substrate; fiber optics; fluorescence; periodontium disorder; stromelysin

The treatment of periodontitis is handicapped by the lack of simple, chairside diagnostic tests capable of differentiating between sites of active disease and sites which exhibit periodontal breakdown but which may currently be quiescent. In addition, many of the common, clinical diagnostic tests (radiographs, clinical examination) require subjective interpretation of data. Studies have shown that different interpretations of the same data can have dramatic effects on decisions which dictate treatment. Proteases in the extracellular matrix have been associated with periodontal disease. Many studies have associated active neutrophil collagenase with tissue destruction and have implicated polymorphonuclear leukocytes as being an important, if not the major, contributor to the acute disease process. Other studies have indicated that specific proteases may determine the outcome of surgical, bone-regenerative procedures. These data indicate that types and amounts of proteases may be an important diagnostic indicator of tissue health. The purpose of this proposal is to obtain initial funding to develop a new, objective diagnostic instrument that will allow simultaneous quantitation of multiple proteases within a single periodontal pocket using chemical fiber optic sensors. Moreover, this pilot study will be used to establish instrumentation which can be used in combination with specific substrates to measure literally hundreds of different compounds simultaneously with the same fiber optic probe. The instrumentation may, in the future, be coupled with technology for enzyme-linked immunosorbance assays (ELISA). This approach could potentially be adapted to use specific antibodies and chemiluminescence to detect and quantitate virtually any compound and compare concentrations of different compounds within the same periodontal pocket. The device could also be used to assay secretions in salivary ducts or from a variety of wounds. The applicability is, therefore, not solely limited to dentistry and the device would be important both for clinical diagnostics and as a research tool.

牙周炎的治疗缺乏能够区分活性疾病部位和表现出牙周崩溃但目前可能是静止的部位的部位的简单，椅子诊断测试的残障。 此外，许多常见的临床诊断测试（X光片，临床检查）需要对数据进行主观解释。 研究表明，对相同数据的不同解释可能会对决定治疗的决策产生巨大影响。 细胞外基质中的蛋白酶与牙周疾病有关。 许多研究已经将活性中性胶原酶与组织破坏相关联，并将多形核白细胞视为重要的（即使不是主要的）急性疾病过程的重要人物。 其他研究表明，特定的蛋白酶可能决定手术，骨再生程序的结果。 这些数据表明，蛋白酶的类型和量可能是组织健康的重要诊断指标。 该提案的目的是获得初始资金，以开发一种新的客观诊断仪器，该工具将允许使用化学光纤传感器在单个牙周口袋内同时定量多个蛋白酶。 此外，该试验研究将用于建立仪器，可以与特定的底物结合使用，以与相同的光纤探针同时测量数百种不同的化合物。 将来，该仪器可能会与用于酶联免疫吸收测定法（ELISA）的技术相结合。 这种方法可能会适应使用特定的抗体和化学发光来检测和定量同一牙周口袋内不同化合物的浓度几乎所有化合物。 该设备也可以用于分泌唾液管或各种伤口中的分泌物。 因此，适用性不仅限于牙科，而且该设备对于临床诊断和作为研究工具都很重要。