Core--Transgenic animal

核心--转基因动物

• 批准号: 6396018
• 负责人: PAK H CHAN
• 金额: $ 18.53万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-03-01 至 2001-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6396018
• 关键词: animal colony; biomedical facility; free radical oxygen; gel electrophoresis; gene targeting; genetically modified animals; laboratory mouse; laboratory rat; superoxide dismutase

The purpose of this Core is to make available transgenic and knockout mutants for Projects 1 and 3 of the program. One particular strength of this Transgenic Animal Core is that Dr. Chan (Core Leader) will provide his expertise in the use of superoxide dismutase, transgenic and knockout mutant mice, as well as oxygen radical-related biochemistry and molecular biology to the other projects, including 1 and 3. In addition, this Core will provide our expertise in molecular analysis of gene expression (PCR, RT-PCR) and gel electrophoresis for enzyme activities of CuZnSOD and MnSOD in mice and rats. Thus, this Transgenic Animal Core provides a unique vehicle for the projects to achieve their research goals with various transgenic and knockout animals.

该核心的目的是为该计划的项目 1 和 3 提供转基因和敲除突变体。该转基因动物核心的一个特殊优势是陈博士（核心领导者）将为其他项目提供他在使用超氧化物歧化酶、转基因和基因敲除突变小鼠以及氧自由基相关生物化学和分子生物学方面的专业知识，包括 1 和 3。此外，该核心将提供我们在基因表达分子分析（PCR、RT-PCR）以及小鼠和大鼠 CuZnSOD 和 MnSOD 酶活性凝胶电泳方面的专业知识。因此，这种转基因动物核心为项目提供了一种独特的工具，以通过各种转基因和基因敲除动物实现其研究目标。