Core--Transgenic animal

核心--转基因动物

基本信息

批准号：
7062927
负责人：
PAK H CHAN
金额：
$ 20万
依托单位：
STANFORD UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/7062927
关键词：
animal breeding animal colony astrocytes biomedical facility enzyme activity free radical oxygen gel electrophoresis gene expression genetically modified animals laboratory mouse laboratory rat molecular biology neurons superoxide dismutase tissue /cell culture vascular endothelium

项目摘要

The purpose of this core is to make available various transgenic and knockout mutants for Projects by Giffard, Steinberg, and Chan. One particular strength of this Transgenic Animal Core is that Dr. Chan (Core Leader) will provide his expertise to the other projects in the use of superoxide dismutases, in both transgenic and knockout mutant mice, as well as oxygen radical-related biochemistry and molecular biology. In addition, Dr. Chan will provide his expertise in primary cell culture of neurons, astrocytes, and endothelial cells from rodents. This Core will serve as a centralized facility for set-up, characterization and quality control of these primary cell cultures from wild-type animals, as well as SOD1, SOD2, and other transgenic and knockout mutant mice. This Core will also provide expertise in the molecular analysis of gene expression (PCR, RT-PCR), gel electrophoresis, enzymatic activity of CuZnSOD and MnSOD and biochemical assays in mice and in primary cell cultures. In addition, the Core will be used to provide technical assistance in breeding the various transgenic and knockout mice for the various projects of this program. Thus, the Transgenic Animal Core provides a unique vehicle for the projects to achieve the research goals employing various transgenic and knockout animals.

该核心的目的是为 Giffard、Steinberg 和 Chan 的项目提供各种转基因和敲除突变体。该转基因动物核心的一个特殊优势是，陈博士（核心领导者）将为其他项目提供他在转基因和基因敲除突变小鼠中使用超氧化物歧化酶的专业知识，以及氧自由基相关的生物化学和分子生物学方面的专业知识。生物学。此外，陈博士还将提供他在啮齿动物神经元、星形胶质细胞和内皮细胞原代细胞培养方面的专业知识。该核心将作为一个集中设施，用于对野生型动物以及 SOD1、SOD2 和其他转基因和敲除突变小鼠的原代细胞培养物进行设置、表征和质量控制。该核心还将提供基因表达分子分析（PCR、RT-PCR）、凝胶电泳、CuZnSOD 和 MnSOD 酶活性以及小鼠和原代细胞培养物生化测定方面的专业知识。此外，该核心还将用于为该计划的各个项目培育各种转基因和基因敲除小鼠提供技术援助。因此，转基因动物核心为项目提供了一种独特的工具，以实现利用各种转基因和基因敲除动物的研究目标。