Core--Transgenic animal

核心--转基因动物

基本信息

批准号：
7062927
负责人：
PAK H CHAN
金额：
$ 20万
依托单位：
STANFORD UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/7062927
关键词：
animal breeding animal colony astrocytes biomedical facility enzyme activity free radical oxygen gel electrophoresis gene expression genetically modified animals laboratory mouse laboratory rat molecular biology neurons superoxide dismutase tissue /cell culture vascular endothelium

项目摘要

The purpose of this core is to make available various transgenic and knockout mutants for Projects by Giffard, Steinberg, and Chan. One particular strength of this Transgenic Animal Core is that Dr. Chan (Core Leader) will provide his expertise to the other projects in the use of superoxide dismutases, in both transgenic and knockout mutant mice, as well as oxygen radical-related biochemistry and molecular biology. In addition, Dr. Chan will provide his expertise in primary cell culture of neurons, astrocytes, and endothelial cells from rodents. This Core will serve as a centralized facility for set-up, characterization and quality control of these primary cell cultures from wild-type animals, as well as SOD1, SOD2, and other transgenic and knockout mutant mice. This Core will also provide expertise in the molecular analysis of gene expression (PCR, RT-PCR), gel electrophoresis, enzymatic activity of CuZnSOD and MnSOD and biochemical assays in mice and in primary cell cultures. In addition, the Core will be used to provide technical assistance in breeding the various transgenic and knockout mice for the various projects of this program. Thus, the Transgenic Animal Core provides a unique vehicle for the projects to achieve the research goals employing various transgenic and knockout animals.

该核心的目的是为Giffard，Steinberg和Chan提供各种转基因和淘汰突变体。这种转基因动物核心的一个特殊优势是，陈（核心领导者）将在转基因和基因敲除突变小鼠以及氧自由基的生物化学和分子生物学的其他项目中为其他项目提供他的专业知识。此外，Chan博士将在啮齿动物的神经元，星形胶质细胞和内皮细胞的原代细胞培养中提供他的专业知识。该核心将作为来自野生型动物的这些原代细胞培养物以及SOD1，SOD2以及其他转基因和敲除突变小鼠的原代细胞培养物的设置，表征和质量控制的集中式设施。该核心还将在小鼠和原发性细胞培养物中的基因表达（PCR，RT-PCR），Cuznsod和Mnsod的酶活性以及生化测定方面提供专业知识。此外，核心将用于为该计划的各种项目繁殖各种转基因和淘汰小鼠提供技术援助。因此，转基因动物核心为项目提供了独特的工具，以实现采用各种转基因和淘汰动物的研究目标。