IN VIVO MRM OF TRANSGEN ANIMAL MODEL OF CEREBRAL AMYLOID ANGIOPATHY: HEMORRHAGE

脑淀粉样血管病转基因动物模型的体内 MRM：出血

• 批准号: 6348187
• 负责人: PATRICK M SULLIVAN
• 金额: $ 0.38万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-01 至 2001-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6348187
• 关键词: biomedical resource; nuclear magnetic resonance spectroscopy; plants

The number of reports within the literature describing plant response to Al3+ exposure reflects a widespread and growing concern about the effects of Al3+ release. Release of this potentially toxic element can occur through several processes, including profound environmental disturbance such as coal mining or "acid rain". We propose to test the hypothesis that the a symbioticfungus Pisolithus tinctorius growing on pine roots will increase tolerance of seedlings to Al3+, and thus will increase plant survival following toxin exposure. New techniques including magnetic resonance imaging (MRI as is used for medical diagnostics) and NMR spectroscopy of intact plants will be used to assess the effect of the fungus and toxin exposure on root growth, turnover and architecture and chemistry. Ultrastructural changes and localization of Al and P will also be determined. Seedlings inoculated with P. tinctorius and planted on two mine sites will also be studied, with the goal of confirming a beneficial effect of the symbiotic fungus in promoting ecosystem restoration. Specific contributions include: 1) dynamic views of root growth, architecture and turnover as a function of Al3+ exposure and mycorrhizal colonization, 2) determination of Al chemistry within intact roots, 3) determination of the effects of Al3+ on P metabolism 4) ultrastructural information and elemental localization, 5) correlative information from field trees, 6) confirmation of the role of P. tinctorius in providing protection to the host against Al3+ toxicity, and 7) further development of 27Al NMR spectroscopic technology which can be extended to additional studies.

文献中描述植物的报告数量 对AL3+暴露的反应反映了广泛而日益严重的关注 关于AL3+释放的影响。 释放这种潜在有毒 元素可以通过多个过程发生，包括深刻 环境干扰，例如煤矿开采或“酸雨”。 我们 提议检验A s sembioticfungus pisolithus的假设 在松根上生长的Tinctorius将增加幼苗的耐受性 到Al3+，因此会在毒素后增加植物生存 接触。 包括磁共振成像在内的新技术（MRI AS 用于医学诊断）和完整植物的NMR光谱法 将用于评估真菌和毒素暴露对 根生长，周转，建筑和化学。 超微结构 还将确定AL和P的变化和定位。 接种tinctorius并种植在两个矿场上的幼苗 还将研究，目的是确认有益的效果 共生真菌促进生态系统恢复。 具体的 贡献包括：1）根生长，体系结构的动态视图 并且营业额是AL3+暴露和菌根的函数 定植，2）确定完整根部的Al化学，3） 确定Al3+对P代谢4的影响4） 超微结构信息和元素定位，5）相关性 来自田间树的信息，6）确认P的作用。 tinctorius为宿主提供防止AL3+毒性的保护， 7）进一步开发27AL NMR光谱技术 可以扩展到其他研究。