A HUMAN APOE MOUSE MODEL OF ALZHEIMER?S DISEASE

阿尔茨海默病的人猿小鼠模型

基本信息

批准号：
8171625
负责人：
PATRICK M SULLIVAN
金额：
$ 0.55万
依托单位：
DUKE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-07-01 至 2011-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8171625
关键词：
Alzheimer&apos s Disease Alzheimer&apos s disease model Animals Apolipoprotein E Behavioral Brain Brain imaging Cerebral hemisphere hemorrhage Computer Retrieval of Information on Scientific Projects Database Dementia Functional disorder Funding Gliosis Grant Human Institution Lateral Life Mus Research Research Personnel Resources Source Synapses United States National Institutes of Health aged apolipoprotein E-3 apolipoprotein E-4 base lateral ventricle mouse model

Alzheimer&apos s Disease Alzheimer&apos s disease model Animals Apolipoprotein E Behavioral Brain Brain imaging Cerebral hemisphere hemorrhage Computer Retrieval of Information on Scientific Projects Database Dementia Functional disorder Funding Gliosis Grant Human Institution Lateral Life Mus Research Research Personnel Resources Source Synapses United States National Institutes of Health aged apolipoprotein E-3 apolipoprotein E-4 base lateral ventricle mouse model

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项目摘要

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The mice involved in this project are 21-23 month old human apoE3 and E4 targeted replacement mice on a C57Bl6 background. We have observed enlarged lateral ventricles in the brains of 18 mo. old human apoE4 mice (compared to apoE3 mice) based on histological sections and would like to confirm using brain imaging on live animals. We have several other indicators of Alzheimer's-like dementia in the aged apoE4 mice such as behavioral and electrophysiological deficits. Furthermore, the apoE4 mice have significantly higher number of intracerebral hemorrhages, gliosis and immunocytochemical markers of synaptic dysfunction. If we are able to demonstrate significant enlargement of the lateral (or other) ventricles in these mice vs controls, it would be a very important discovery, strengthening the evidence for these mice to be used as models of AD.

该副本是利用众多研究子项目之一由NIH/NCRR资助的中心赠款提供的资源。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构是对于中心，这不一定是调查员的机构。参与该项目的小鼠是在C57BL6背景下具有21-23个月的Human ApoE3和E4靶向替换小鼠。我们已经观察到18个月大脑中的侧心肿大。基于组织学部分的旧人APOE4小鼠（与APOE3小鼠相比），并希望使用活动物的脑成像确认。我们还有其他几个指标的阿尔茨海默氏症痴呆症在老年apoe4小鼠中，例如行为和电生理缺陷。此外，APOE4小鼠具有突触功能障碍的脑出血，神经胶质病和免疫细胞化学标记的数量明显更高。如果我们能够证明这些小鼠与对照中的侧（或其他）心室的显着扩大，那将是一个非常重要的发现，可以加强这些小鼠用作AD模型的证据。