CNS AUTONOMIC PATHWAYS AND GASTROINTESTINAL FUNCTION

中枢神经系统自主通路和胃肠道功能

• 批准号: 6176574
• 负责人: PAMELA J HORNBY
• 金额: $ 17.71万
• 依托单位: LSU HEALTH SCIENCES CENTER
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-08-01 至 2002-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6176574
• 关键词: afferent nerve; autonomic nervous system; brain regulatory center; central nervous system; central neural pathway /tract; cholinergic agents; cisapride; dorsal motor nucleus; endothelin; gamma aminobutyrate; gastrointestinal function; gastrointestinal motility /pressure; histology; immunocytochemistry; innervation; laboratory rat; microinjections; neural information processing; nitric oxide; serotonin; thyrotropin releasing hormone; vagus nerve

DESCRIPTION: The overall aim is identified as the intention to test whether, in the dorsal vagal complex, neuroactive substances from neuronal and humoral sources ultimately mediate gastric motor excitation or inhibition due to receptor mediated interactions and due to their effects on nitrergic and cholinergic vagal outflow to the stomach. Given this overall conception the investigator proposes two specific aims each with three subpoints. The hypothesis of specific aim 1 is that in the dorsal vagal complex gastric excitatory and inhibitory neuronal inputs independently control basal gastric motility through both nitrergic and cholinergic outflow to the stomach. The second specific aim is to test the hypothesis that selected humoral and therapeutic agents and afferant input act in the DVC to mediate gastric effects dependent on neuronal influences during both basal and reflex stimulated changes in gastric motor function. Sub-aim 2a is do animals rendered unresponsive to nitric oxide TRH and serotonin receptor subtype activation in the dorsal vagal complex respond with expected increases in gastric motor function after endothelin-1 or cisapride. Sub-aim 2b: does cisapride change basal gastric motor function by acting in the DVC and can it act there to alter ET-1 evoked gastric excitation or gastric relaxation in response to colonic distension reflex-evoked gastric changes. Two experiments are proposed for this sub-aim which involve giving cisapride microinjected into the DVC in animals who have been anesthetized and with the dorsal medulla exposed.

描述：总体目标被确定为测试意图 无论是在背部迷走络合物中，神经元的神经活性物质 和体液来源最终介导胃运动激发或 由于受体介导的相互作用而引起的抑制作用以及它们对 硝化和胆碱能的迷走神经流向胃。 总体而言 概念研究者提出了两个具体目标，每个目标都有三个 子点。 特定目的1的假设是在背迷走神经中 复杂的胃兴奋性和抑制性神经元输入独立 通过硝化和胆碱能控制基底胃运动 流向胃。 第二个具体目的是检验假设 选定的体液和治疗剂和传入的输入法 DVC介导胃作用取决于神经元的影响 基础和反射刺激了胃运动功能的变化。 sub-aim 2a 动物对一氧化氮TRH和5-羟色胺没有反应而产生反应吗？ 背部迷走神经复合物中受体亚型激活与 内皮素-1或 西萨普里德。 Sub-aim 2b：Cisapride会改变基础胃运动功能 通过在DVC中作用，可以在此作用以改变ET-1唤起胃 响应结肠扩张的激发或胃松弛 反射引起的胃变化。 为此提出了两个实验 sub-aim，涉及将sisapride微注射到动物中的DVC中 被麻醉的，背髓暴露了。