POST-TRANSCRIPTIONAL CONTROL OF FLAGELLAR FILAMENT

鞭毛丝的转录后控制

• 批准号: 6226487
• 负责人: KELLY T HUGHES
• 金额: $ 18.77万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-03-01 至 2005-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6226487
• 关键词: Salmonella typhimurium; bacterial genetics; bacterial proteins; biological signal transduction; flagellum; gene mutation; genetic regulation; genetic regulatory element; secretion; transcription factor

DESCRIPTION: The development of the flagellar organelle in Salmonella typhimurium is a complex process in which the assembly pathway is intimately coupled to the regulation of gene expression and levels of subunit proteins to be assembled. The transcription of the flagellar filament protein is coupled to the completion of a basal structure, the hook-basal body. We have obtained evidence of post-transcriptional control of both the filament (FliC) and hook (FIgE) subunit proteins in response to flagellar assembly. The process of extracellular assembly is mediated by secretion through a mechanism commonly used to secrete pathogenecity proteins into the cytosol of eukaryotic host cells, the type III secretion system. We have evidence that the F1gE hook component of the bacterial flagella is regulated at the post-transcriptional level in response to the assembly of the cytoplasmic component of the flagellar-specific-type III secretion apparatus, the C-rod. We also have evidence that the intracellular level of FliC filament protein is regulated by a post-transcriptional mechanism in addition to the well-characterized transcriptional control. This research proposed here will determine the mechanism (i.e., trans-acting proteins and cis-acting mRNA signals) which mediate the regulation of FlgE and F1iC levels in response to flagellar assembly. This research will also identify the cis- and trans-acting factors or signals that mediate the regulation of FlgE and FliC levels

描述：沙门氏菌中鞭毛细胞器的开发 鼠伤寒是一个复杂的过程，其中组件途径密切相关 结合基因表达和亚基蛋白水平的调节 组装。鞭毛丝蛋白的转录耦合到 基础结构的完成，即钩子体。我们已经获得了 丝（FLIC）和钩子的转录后控制的证据 （FIGE）响应鞭毛组件的亚基蛋白。过程 细胞外组装是通过通常通过一种机制介导的 用于将病原体蛋白分泌到真核宿主的细胞质中 细胞，III型分泌系统。我们有证据表明F1GE钩子 细菌鞭毛的成分受到转录后的调节 响应于组装的细胞质成分的水平 鞭毛特异性型III分泌设备，C-Rod。我们也有 证据表明细胞内丝状细丝蛋白受到调节 除了良好的特征之外，转录后机制 转录控制。此处提出的这项研究将确定 机理（即反式作用蛋白和顺式作用mRNA信号） 响应FLGE和F1IC水平的调节，以响应鞭毛 集会。这项研究还将确定顺式和跨性别因素或 介导FLGE和FLIC水平调节的信号