ACQUISITION OF AN 800 MHZ NMR SPECTROMETER

购买 800 MHZ 核磁共振波谱仪

• 批准号: 6288315
• 负责人: THOMAS L JAMES
• 金额: $ 50万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-03-15 至 2003-09-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6288315
• 关键词: biomedical equipment purchase; nuclear magnetic resonance spectroscopy

We propose the acquisition of an 800 MHz NMR spectrometer that will serve the research community of UCSF primarily with some service for researchers on other University of California campuses in Northern California. The research proposed for the new instrument will improve our capability to use multidimensional NMR experiments for determining high-resolution structures in solution primarily of proteins, nucleic acids, and complexes entailing proteins and nucleic acids, as a means of elucidating biological function. The additional sensitivity and resolution, as well as enhanced utilization of residual dipolar coupling measurements and pulse sequences incorporating transverse relaxation optimized spectroscopy (TROSY), afforded by the new instrument will not only increase through-put in structure determination, but it will permit determination of resonance assignments in regions which are currently ambiguous, enable additional structural restraints to be determined leading to higher quality structures, enable some structure determinations which have not been possible so far due to aggregation problems, and extend our ability to determine structures of biopolymers with molecular mass >30 kilodaltons.

我们建议收购800 MHz NMR光谱仪，该光谱仪将主要为加利福尼亚大学北加州其他大学校园的研究人员提供一些服务。提出的针对新仪器的研究将提高我们使用多维NMR实验的能力，以确定主要是蛋白质，核酸和络合物的溶液中的高分辨率结构，这是蛋白质和核酸的，作为阐明生物学功能的一种手段。额外的灵敏度和分辨率，以及增强的残留偶极耦合测量测量和脉冲序列的利用，包括新仪器提供的横向松弛优化光谱（TROSY）不仅可以在结构确定中逐步提高，而且还可以确定。当前模棱两可的区域中的共振分配能够确定额外的结构限制，从而导致更高质量的结构，从而使某些由于聚集问题而无法实现的结构确定，并扩展了我们确定分子质量结构的能力> 30公斤。