PE BIOSYSTEMS VOYAGER DE MALDI-TOF

PE 生物系统 Voyager DE MALDI-TOF

• 批准号: 6291328
• 负责人: KENNETH G MANN
• 金额: $ 18万
• 依托单位: UNIVERSITY OF VERMONT & ST AGRIC COLLEGE
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-04-15 至 2002-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6291328
• 关键词: biomedical equipment purchase; mass spectrometry; matrix assisted laser desorption ionization

This request is for funds to purchase a Voyager-DE Biospectrometry Workstation. This system uses matrix-assisted of proteins, peptide and oligonucleotide. The power of this instrument lies in the delayed extraction (DE) technology. Significant mass resolution is obtained by reducing the background and increasing the signal to noise ratio. Therefore analyses of complex protein matures from various sources and fragile molecules, such as DNA, can be accomplished with great accuracy. Because of the decreased background the software is able to narrow the parameters and significantly reduce the number of masers necessary to make valid assignments. The software also allows identification of proteins by searching a virtual digestion database. This system is ideal for multiple operators and projects beaus of its versatility, spread and ease of operation. Our specific applications include: C-terminal and N-terminal protein and oligonucleotide sequencing, identifying unknown proteins and determining post-transnational modifications. Samples can be extracted from such sources as gels, PVDF membranes, TLC plates, and/or Biacore chips. The fulfillment of the specific aims described in detail in the twelve research proposals would be facilitated by the addiction of a Voyager-DE MALDI-TOF

此请求是要购买Voyager-DE生物光谱法工作站的资金。该系统使用蛋白质，肽和寡核苷酸的基质辅助。该仪器的力量在于延迟提取（DE）技术。通过减少背景并增加信号与噪声比，可以获得大量质量分辨率。因此，可以非常准确地完成对各种来源和脆弱分子（例如DNA）成熟的复杂蛋白质成熟的分析。由于背景下降，软件能够缩小参数，并显着减少进行有效任务所需的MASER数量。该软件还可以通过搜索虚拟消化数据库来识别蛋白质。该系统非常适合多个运营商和项目的多功能性，传播和易于操作。我们的特定应用包括：C末端和N末端蛋白和寡核苷酸测序，鉴定未知的蛋白质并确定翻译后修饰。可以从诸如凝胶，PVDF膜，TLC板和/或Biacore芯片等来源中提取样品。十二个研究建议中详细描述的具体目的的实现将有助于Voyager-de Maldi-Tof的成瘾