Oral spirochetes: molecular genetic analysis

口腔螺旋体：分子遗传学分析

• 批准号: 6323961
• 负责人: HOWARD K KURAMITSU
• 金额: $ 20.28万
• 依托单位: STATE UNIVERSITY OF NEW YORK AT BUFFALO
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-02-20 至 2005-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6323961
• 关键词: Bacteroides gingivalis; Fusobacterium nucleatum; Spirochaetales; Treponema; Treponema pallidum; bacteria infection mechanism; bacterial genetics; gene expression; genetic mapping; genetic regulation; host organism interaction; infection; molecular film; molecular genetics; mutant; oral bacteria; transcytosis; transfection /expression vector; virulence

DESCRIPTION: This application will continue to examine the molecular basis for the virulence of spirochetes. Utilizing Treponema denticola mutants constructed in the previous grant period, the respective roles of motility, chemotaxis, and protease activity in the formation of biofilms will be investigated. In addition, a RNA differential display approach will be used to identify genes, which are involved in colonization of solid surfaces, preformed biofilms of Porphyromonas gingivalis and Fusobacterium nucleatum, and host cell surfaces. These approaches will help to define the mechanisms, which oral spirochetes utilize in colonizing the subgingival margin. Furthermore, since penetration of tissue barriers by spirochetes appears to be an important virulence factor of these organisms, the molecular basis for this property will be examined in T denticola. Using the HUVEC transcytosis system, genes of the spirochete, which are involved in tissue penetration, will be identified using genetic approaches. It will then be of interest to determine if the syphilitic organism T pallidum utilizes a homologous set of genes to invade tissue. In addition, if the later organism utilizes species-specific invasion genes, the hypothesis that oral spirochetes, which are related to T. pallidum, may play a role in periodontitis will be examined. These approaches should increase understanding of the molecular basis for the pathogenicity of spirochetes.

描述：本申请将继续研究以下物质的分子基础： 螺旋体的毒力。利用构建的齿垢密螺旋体突变体 在上一个资助期内，运动性、趋化性和 将研究生物膜形成中的蛋白酶活性。在 此外，RNA差异显示方法将用于识别基因， 参与固体表面的定殖，预形成的生物膜 牙龈卟啉单胞菌和具核梭杆菌以及宿主细胞表面。 这些方法将有助于定义口腔螺旋体的机制 用于定植龈下边缘。此外，由于渗透 螺旋体的组织屏障似乎是重要的毒力因子 这些生物体，该特性的分子基础将在 T 中进行检查 齿垢。使用 HUVEC 转胞吞系统，螺旋体的基因， 参与组织渗透，将通过基因鉴定 接近。然后确定梅毒生物体是否 梅毒螺旋体利用一组同源基因来侵入组织。另外，如果 后来的生物体利用物种特异性的入侵基因，假设 与梅毒螺旋体相关的口腔螺旋体可能在 将检查牙周炎。这些方法应该增加理解 螺旋体致病性的分子基础。