Photodynamic Therapy for Endodontic Disinfection

牙髓消毒的光动力疗法

• 批准号: 7585800
• 负责人: NIKOLAOS SOUKOS
• 金额: $ 40.85万
• 依托单位: ADA FORSYTH INSTITUTE, INC.
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2010-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7585800
• 关键词: Actinomyces israelii; Affect; Aftercare; Anti-Bacterial Agents; Antimicrobial Effect; Apoptosis; Appointment; Bacteria; Biological Assay; Cell Proliferation; Cell Survival; Cells; Clinical Trials; Colony-forming units; Complex; DNA; Debridement; Dental Pulp; Dental Pulp Necrosis; Dentin; Disinfection; Dose; Effectiveness; Electromagnetics; Endodontics; Enterococcus faecalis; Enzyme-Linked Immunosorbent Assay; Excision; Exposure to; Failure; Fibroblasts; Filament; Fluorescence; Fluorescent Probes; Fusobacterium nucleatum; Genomics; Goals; Growth; Human; In Vitro; Induction of Apoptosis; Infection; Invaded; Irrigation; Laser Scanning Confocal Microscopy; Laser Scanning Microscopy; Left; Lesion; Life; Light; Lighting; Mechanics; Microbial Biofilms; Modeling; Mono-S; Necrosis; Neutral Red; Operative Surgical Procedures; Order Spirochaetales; Organism; Osteoblasts; Periodontal Ligament; Pharmaceutical Preparations; Phase; Photochemotherapy; Photosensitizing Agents; Phototherapy; Plant Roots; Polymerase Chain Reaction; Porphyromonas; Prevotella intermedia; Procedures; Propidium Diiodide; Public Health; Pulp Canals; Research; Research Personnel; Residual state; Retreatment; Root Canal Obturation; Safety; Scanning Electron Microscopy; Sodium Hypochlorite; Staining method; Stains; Sterile coverings; Structure; Study Section; Surface; System; Techniques; Testing; Therapeutic; Time; Tissues; Tooth structure; Treatment Failure; Visible Radiation; absorption; antimicrobial; bone cell; cell killing; energy density; fungus; instrumentation; killings; microorganism; periapical; programs; retinal rods; skills; success

Endodontic failures are caused by the proliferation of residual bacteria that are left behind within the root canal due to the complexity of the root canal system that makes complete debridement with instrumentation and irrigation alone almost impossible. Our long-term goal is to develop photodynamic therapy (PDT) as an adjunctive technique to eliminate residual root canal bacteria after instrumentation and irrigation with sodium hypochlorite (NaOCI). The specific hypothesis to be tested by this research is that visible light can achieve a rapid and complete killing of residual root canal microorganisms after their sensitization with a photoactive drug. The specific aims are to: 1. Develop mono- and multi-species biofilms with microorganisms that are common in endodontic failures and/or infections on the surface as well as in the dentinal tubules of root canal systems of extracted teeth. We will (i) characterize these biofilms by scanning electron microscopy and confocal scanning laser microscopy and we will (ii)analyze them using checkerboard DNA-DNA hybridization, polymerase chain reaction (PCR) assays and culture. 2. Investigate the photodestruction of root canal biofilms in vitro. We will (i) establish the parameters of light and photosensitizers for the complete elimination of root canal microbial biofilms, (ii)evaluate bacterial killing, and (iii)assess the distribution of cell killing within the biofilms. 3. Assess the safety of photodynamic therapy. We will (i) determine the viability of human periodontal ligament and bone cells after treatment, (ii)determine the therapeutic window whereby microorganisms can be inactivated without affecting host cell viability, and iii) investigate the induction of apoptosis. 4. Evaluate the antimicrobial effects of photodynamic therapy in human teeth ex vivo. We will use freshly extracted infected human teeth to evaluate the effect of photodynamic therapy on residual bacteria in the root canal system after complete instrumentation and irrigation with NaOCI. Relevance to public health: More than 2 million root canal retreatments due to residual microorganisms are performed yearly in the US. Photodynamic therapy may be an adjunctive procedure to kill residual root canal bacteria after their sensitization with a photoactive drug and their subsequent exposure to light.

牙髓病失败是由于牙根内残留细菌的增殖引起的 由于根管系统的复杂性，需要使用器械进行彻底清创 仅靠灌溉几乎是不可能的。我们的长期目标是开发光动力疗法（PDT）作为一种 使用仪器和钠冲洗后消除残留根管细菌的辅助技术 次氯酸盐（NaOCI）。本研究要检验的具体假设是可见光可以实现 在光敏剂致敏后，快速彻底杀死残留的根管微生物 药品。具体目标是： 1. 使用牙髓失败中常见的微生物开发单物种和多物种生物膜 和/或拔牙的根管系统表面以及牙本质小管的感染。 我们将 (i) 通过扫描电子显微镜和共焦扫描激光来表征这些生物膜 显微镜检查，我们将 (ii) 使用棋盘 DNA-DNA 杂交、聚合酶链对其进行分析 反应（PCR）测定和培养。 2. 体外研究根管生物膜的光破坏作用。我们将 (i) 建立光的参数 和光敏剂，用于完全消除根管微生物生物膜，（ii）评估细菌 杀伤，以及（iii）评估生物膜内细胞杀伤的分布。 3.评估光动力疗法的安全性。我们将 (i) 确定人类牙周的活力 治疗后的韧带和骨细胞，(ii)确定微生物可以利用的治疗窗口 在不影响宿主细胞活力的情况下被灭活，并且iii)研究细胞凋亡的诱导。 4. 评价光动力疗法对人牙齿离体的抗菌作用。我们将使用新鲜的 拔出受感染的人类牙齿以评估光动力疗法对牙齿中残留细菌的效果 完成仪器安装并用 NaOCI 冲洗后的根管系统。 与公共健康的相关性：超过 200 万次根管治疗因残留微生物而进行 每年在美国演出。光动力疗法可能是杀死残留根管的辅助手术 细菌被光活性药物致敏并随后暴露在光下。

项目成果

Endodontic photodynamic therapy ex vivo.

• DOI: 10.1016/j.joen.2010.10.008
• 发表时间: 2011-02
• 期刊: Journal of endodontics
• 影响因子: 4.2
• 作者: Ng R;Singh F;Papamanou DA;Song X;Patel C;Holewa C;Patel N;Klepac-Ceraj V;Fontana CR;Kent R;Pagonis TC;Stashenko PP;Soukos NS
• 通讯作者: Soukos NS

Nanoparticle-based endodontic antimicrobial photodynamic therapy.

• DOI: 10.1016/j.joen.2009.10.011
• 发表时间: 2010-02
• 期刊: Journal of endodontics
• 影响因子: 4.2
• 作者: Pagonis TC;Chen J;Fontana CR;Devalapally H;Ruggiero K;Song X;Foschi F;Dunham J;Skobe Z;Yamazaki H;Kent R;Tanner AC;Amiji MM;Soukos NS
• 通讯作者: Soukos NS