Photodynamic Therapy for Endodontic Disinfection

牙髓消毒的光动力疗法

• 批准号: 7585800
• 负责人: NIKOLAOS SOUKOS
• 金额: $ 40.85万
• 依托单位: ADA FORSYTH INSTITUTE, INC.
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2010-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7585800
• 关键词: Actinomyces israelii; Affect; Aftercare; Anti-Bacterial Agents; Antimicrobial Effect; Apoptosis; Appointment; Bacteria; Biological Assay; Cell Proliferation; Cell Survival; Cells; Clinical Trials; Colony-forming units; Complex; DNA; Debridement; Dental Pulp; Dental Pulp Necrosis; Dentin; Disinfection; Dose; Effectiveness; Electromagnetics; Endodontics; Enterococcus faecalis; Enzyme-Linked Immunosorbent Assay; Excision; Exposure to; Failure; Fibroblasts; Filament; Fluorescence; Fluorescent Probes; Fusobacterium nucleatum; Genomics; Goals; Growth; Human; In Vitro; Induction of Apoptosis; Infection; Invaded; Irrigation; Laser Scanning Confocal Microscopy; Laser Scanning Microscopy; Left; Lesion; Life; Light; Lighting; Mechanics; Microbial Biofilms; Modeling; Mono-S; Necrosis; Neutral Red; Operative Surgical Procedures; Order Spirochaetales; Organism; Osteoblasts; Periodontal Ligament; Pharmaceutical Preparations; Phase; Photochemotherapy; Photosensitizing Agents; Phototherapy; Plant Roots; Polymerase Chain Reaction; Porphyromonas; Prevotella intermedia; Procedures; Propidium Diiodide; Public Health; Pulp Canals; Research; Research Personnel; Residual state; Retreatment; Root Canal Obturation; Safety; Scanning Electron Microscopy; Sodium Hypochlorite; Staining method; Stains; Sterile coverings; Structure; Study Section; Surface; System; Techniques; Testing; Therapeutic; Time; Tissues; Tooth structure; Treatment Failure; Visible Radiation; absorption; antimicrobial; bone cell; cell killing; energy density; fungus; instrumentation; killings; microorganism; periapical; programs; retinal rods; skills; success

Endodontic failures are caused by the proliferation of residual bacteria that are left behind within the root canal due to the complexity of the root canal system that makes complete debridement with instrumentation and irrigation alone almost impossible. Our long-term goal is to develop photodynamic therapy (PDT) as an adjunctive technique to eliminate residual root canal bacteria after instrumentation and irrigation with sodium hypochlorite (NaOCI). The specific hypothesis to be tested by this research is that visible light can achieve a rapid and complete killing of residual root canal microorganisms after their sensitization with a photoactive drug. The specific aims are to: 1. Develop mono- and multi-species biofilms with microorganisms that are common in endodontic failures and/or infections on the surface as well as in the dentinal tubules of root canal systems of extracted teeth. We will (i) characterize these biofilms by scanning electron microscopy and confocal scanning laser microscopy and we will (ii)analyze them using checkerboard DNA-DNA hybridization, polymerase chain reaction (PCR) assays and culture. 2. Investigate the photodestruction of root canal biofilms in vitro. We will (i) establish the parameters of light and photosensitizers for the complete elimination of root canal microbial biofilms, (ii)evaluate bacterial killing, and (iii)assess the distribution of cell killing within the biofilms. 3. Assess the safety of photodynamic therapy. We will (i) determine the viability of human periodontal ligament and bone cells after treatment, (ii)determine the therapeutic window whereby microorganisms can be inactivated without affecting host cell viability, and iii) investigate the induction of apoptosis. 4. Evaluate the antimicrobial effects of photodynamic therapy in human teeth ex vivo. We will use freshly extracted infected human teeth to evaluate the effect of photodynamic therapy on residual bacteria in the root canal system after complete instrumentation and irrigation with NaOCI. Relevance to public health: More than 2 million root canal retreatments due to residual microorganisms are performed yearly in the US. Photodynamic therapy may be an adjunctive procedure to kill residual root canal bacteria after their sensitization with a photoactive drug and their subsequent exposure to light.

牙髓衰竭是由残留细菌的增殖引起的 由于根管系统的复杂性而导致运河，从而使仪器完全清创术 仅凭灌溉几乎是不可能的。我们的长期目标是开发光动力疗法（PDT） 仪器和钠灌溉后消除残留的根管细菌的辅助技术 次氯酸盐（NAOCI）。这项研究要检验的具体假设是，可见光可以实现 用光活性敏化后，快速而完全完全杀死了残留的根管微生物 药品。具体目的是： 1。开发具有微生物的单物种生物膜，在牙髓衰竭中很常见 和/或表面上的感染以及提取牙齿的根管系统的牙本小管中。 我们将（i）通过扫描电子显微镜和共聚焦激光来表征这些生物膜 显微镜，我们将（ii）使用棋盘DNA-DNA杂交，聚合酶链分析它们 反应（PCR）测定和培养。 2。研究了体外管道生物膜的光电毁灭。我们将（i）建立光的参数 以及用于完全消除根管微生物生物膜的光敏剂，（ii）评估细菌 杀戮和（iii）评估生物膜内细胞杀死的分布。 3。评估光动力疗法的安全性。我们将（i）确定人类牙周的生存能力 治疗后韧带和骨细胞，（ii）确定微生物可以的治疗窗口 灭活而不会影响宿主细胞的生存力，iii）研究凋亡的诱导。 4。评估光动力疗法在人牙中的抗菌作用。我们将新鲜使用 提取受感染的人牙，以评估光动力疗法对残留细菌的影响 完整的仪器和Naoci灌溉后，根管系统。 与公共卫生的相关性：由于残留微生物而导致的200万根管的撤退是 每年在美国表演。光动力疗法可能是杀死残留根管的辅助手术 细菌用光活化药物敏化后，随后暴露于光。

项目成果

Nanoparticle-based endodontic antimicrobial photodynamic therapy.

• DOI: 10.1016/j.joen.2009.10.011
• 发表时间: 2010-02
• 期刊: Journal of endodontics
• 影响因子: 4.2
• 作者: Pagonis TC;Chen J;Fontana CR;Devalapally H;Ruggiero K;Song X;Foschi F;Dunham J;Skobe Z;Yamazaki H;Kent R;Tanner AC;Amiji MM;Soukos NS
• 通讯作者: Soukos NS

Endodontic photodynamic therapy ex vivo.

• DOI: 10.1016/j.joen.2010.10.008
• 发表时间: 2011-02
• 期刊: Journal of endodontics
• 影响因子: 4.2
• 作者: Ng R;Singh F;Papamanou DA;Song X;Patel C;Holewa C;Patel N;Klepac-Ceraj V;Fontana CR;Kent R;Pagonis TC;Stashenko PP;Soukos NS
• 通讯作者: Soukos NS