Embryonic Salivary Gland Development in Drosophila

果蝇胚胎唾液腺发育

• 批准号: 6334460
• 负责人: Steven K. Beckendorf
• 金额: $ 26.81万
• 依托单位: UNIVERSITY OF CALIFORNIA BERKELEY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-01-01 至 2006-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6334460
• 关键词: Drosophilidae; biological signal transduction; cell cycle; cell growth regulation; complementary DNA; developmental genetics; early embryonic stage; embryogenesis; epidermal growth factor; gene expression; gene interaction; gene mutation; genetic screening; growth factor receptors; histogenesis; homeobox genes; laboratory rabbit; nucleic acid sequence; point mutation; protein tyrosine kinase; receptor expression; salivary glands; temperature sensitive mutant; tissue /cell culture; transcription factor

DESCRIPTION: The proposed study is part of a broad interest by the applicant in the genetic mechanisms that establish distinct tissues and organs during embryogenesis, including both the initial instructions that define an organ and the subsequent cell-cell signaling, changes in gene expression, and cellular movements that build the differentiated organ and maintain its identity. Study of salivary gland development in Drosophila embryos has led to a model for the initial determination of cells in the salivary primordium. Salivary gene expression is activated by the homeotic gene Sex combs reduced, and the salivary gland and salivary duct primordia are distinguished from each other by the opposing activities of the EGF receptor signaling pathway and the transcription factor fork head. Nevertheless, neither the effectors nor the targets of EGFR signaling have been defined. Shortly after their determination, salivary glands begin to form by an orderly invagination that begins at the posterior dorsal edge of the placode and continues to the anterior and then ventral parts of the placode. The ducts form by a continuation of the placode invagination to produce the Y-shaped ducts that are much smaller in diameter than the glands. Although this morphogenesis is highly ordered, its genetic control is just beginning to be investigated. There are five Specific Aims for these experiments: 1) identify the spitz-dependent repressors that prevent fkh expression in the pre-duct cells. 2) define the roles of senseless and daughterless. Do they antagonize the effects of the EGFR pathway; 3) determine whether huckebein and wingless interact to initiate and organize salivary invagination; 4) test whether the Tec29 tyrosine kinase regulates cell division in the salivary placode to facilitate salivary morphogenesis; and 5) use a sensitized genetic screen to identify genes required for salivary duct development.

描述：拟议的研究是申请人广泛兴趣的一部分 在期间建立不同组织和器官的遗传机制 胚胎发生，包括定义器官和 随后的细胞 - 细胞信号传导，基因表达的变化和细胞的变化 建立差异化器官并保持其身份的运动。学习 果蝇胚胎中唾液腺发育的发展已导致了一个模型 唾液原基中细胞的初始测定。唾液基因 表达被同源基因性梳子降低而激活，而 唾液腺和唾液管原始彼此区分 EGF受体信号通路和 转录因子叉头。然而，效应子和 已经定义了EGFR信号的目标。决心不久， 唾液腺开始以有序的内陷开始形成 位置的后侧边缘，然后继续前进，然后 位置的腹侧部分。通过延续的座位形成管道 Y型管道的内陷，直径小得多 比腺体。尽管这种形态发生高度有序，但其遗传 控制才刚刚开始研究。有五个特定目标 这些实验：1）识别依赖Spitz的阻遏物，以防止FKH 在导管细胞中的表达。 2）定义毫无意义的角色和 无女。它们会拮抗EGFR途径的影响吗？ 3）确定 Huckebein和Wingless是否相互作用以发起和组织唾液 不知不觉4）测试TEC29酪氨酸激酶是否调节细胞分裂 在唾液斑块中，以促进唾液形态发生； 5）使用 敏化的遗传筛选以识别唾液管所需的基因 发展。