EMBRYONIC SALIVARY GLAND DEVELOPMENT IN DROSOPHILA

果蝇胚胎唾液腺的发育

• 批准号: 2856665
• 负责人: Steven K. Beckendorf
• 金额: $ 21.58万
• 依托单位: UNIVERSITY OF CALIFORNIA BERKELEY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-01-01 至 2000-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2856665
• 关键词: Drosophilidae; biological signal transduction; complementary DNA; developmental genetics; early embryonic stage; epidermal growth factor; eye; gene expression; gene interaction; gene mutation; growth factor receptors; homeobox genes; laboratory rabbit; nucleic acid sequence; phenotype; point mutation; receptor expression; salivary glands; temperature sensitive mutant; tissue /cell culture; transcription factor

DESCRIPTION (Adapted from investigator's Abstract): The proposed study is part of the applicant's broad interest in the genetic mechanisms that establish distinct tissues and organs during embryogenesis, including both the initial instructions that define an organ and the subsequent cell-cell signaling, changes in gene expression, and cellular movements that build the differentiated organ and maintain its identity. The applicant's study of salivary gland development in Drosophila embryos has led to a model for the initial determination of cells in the salivary duct primordia. Salivary gene expression is activated by the homeotic gene Sex combs reduced, and the salivary gland and salivary duct primordia are distinguished from each other by the opposing activities of the EGF receptor signaling pathway and the transcription factor fork head. Shortly after their determination, both the pregland and preduct cells invaginate and within 3 hours become functional salivary glands and ducts. This proposal focuses on the genetic control of the invaginations in these two tissues. Multiple regulatory genes appear to interact to produce the distinctly different pregland and preduct invaginations. The applicant plans to examine the roles of three genes or gene pairs, fork head, Notch/Delta, and X31, that are involved in invagination of the pregland cells and one gene, eyegone, that is required for invagination of the preduct cells. Because the functions of neither X31 nor eyegone have been previously determined, and because both genes appear to be important, not only for salivary development but also for other determinative or morphogenetic events in the embryo, their involvement in other early embryonic invaginations or in specification of the optic primordia will also be studied. The salivary and nonsalivary studies are expected to reinforce each other and lead to a more rapid and deeper understanding of the function of these genes. There are four specific aims for these experiments: (i) to establish the role of fork head in salivary gland invagination; (ii) to determine whether Notch-Delta signaling is important for salivary gland invagination; (iii) to isolate X31 mutations and use them to establish whether this newly identified gene is required for a series of embryonic invaginations; and (iv) to establish the role of the eyegone gene in both salivary duct and eye development.

描述（根据调查员的摘要改编）：拟议的研究是 申请人对遗传机制的广泛兴趣 在胚胎发生过程中建立不同的组织和器官，包括 定义器官和随后的细胞细胞的初始指令 信号传导，基因表达的变化以及构建的细胞运动 区分器官并保持其身份。 申请人的研究 果蝇胚胎中的唾液腺发育已导致了一个模型 唾液管原始细胞的初始测定。 唾液 基因表达被降低的同源基因性梳激活，而 唾液腺和唾液管原始彼此区分 通过EGF受体信号通路的相对活动和 转录因子叉头。 决心后不久 pregland和preepuct细胞起伏，并且在3小时内起作用 唾液腺和管道。 该提案的重点是 这两种组织中的内陷。 多个调节基因似乎 相互作用以产生明显不同的pregland和Preduct 无知。 申请人计划检查三个基因或 涉及的基因对，叉子头，缺口/三角洲和X31 必需 为了使预距细胞的内陷。 因为x31的功能 以前也没有确定Eyegone，并且因为两个基因出现 重要的是，不仅对于唾液发展，而且对于其他 胚胎中的确定性或形态发生事件，它们参与 其他早期的胚胎起伏或视频规范 基本还将研究。 唾液和非盐学研究是 期望互相加强，并导致更快，更深 了解这些基因的功能。 有四个特定目标 对于这些实验：（i）确定叉头在唾液中的作用 腺体不知所措； （ii）确定Notch-Delta信号是否为 对于唾液腺的起伏很重要； （iii）分离x31突变 并使用它们来确定是否需要这种新鉴定的基因 一系列胚胎的内陷； （iv）确定 唾液管和眼睛发育中的Eyegone基因。