REACTIVE SPECIES IN VASCULAR ENDOTHELIAL CELLS EXPOSED TO LOW LEVELS OF ARSENITE

暴露于低水平亚砷酸盐的血管内皮细胞中的反应物质

• 批准号: 6353190
• 负责人: Aaron Barchowsky
• 金额: $ 1.22万
• 依托单位: DARTMOUTH COLLEGE
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-01 至 2002-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6353190
• 关键词: Mammalia; bioengineering /biomedical engineering; biological products; biomedical resource; environmental toxicology; technology /technique

The reduction of Cr(VI) on the skin of living rats was investigated using the technique of in vivo electron paramagnetic resonance (EPR) spectroscopy with a surface coil resonator. Topical application of aqueous solution of Cr(VI) on the skin of rats generated the transient species, Cr(V). Partial removal of the stratum corneum increased the rates of formation and decay of Cr(V), as well as Cr(V) signal intensity. The results demonstrate that skin represents one of the routes for chromium to enter into animals and humans. The generation of reactive Cr(V) species in the reduction of Cr(VI) by the skin may play an important role in the mechanism of Cr(VI)-induced skin cancer. These findings indicate the potential for in vivo EPR spectroscopy to study the metabolism of paramagnetic reactive species in chemical and biochemical reactions occurring in/on the skin of both small and large animals, and possibly humans.

活体大鼠皮肤上 Cr(VI) 的还原 使用体内电子顺磁技术进行研究 带有表面线圈谐振器的共振（EPR）光谱。 专题 Cr(VI)水溶液在大鼠皮肤上的涂抹 生成瞬态物质 Cr(V)。 部分移除 角质层增加了 Cr(V) 的形成和衰变速率， 以及 Cr(V) 信号强度。 结果表明，皮肤 代表铬进入动物体内的途径之一 人类。 还原过程中产生活性 Cr(V) 物种 皮肤的 Cr(VI) 可能在机制中发挥重要作用 Cr(VI) 诱发的皮肤癌。 这些发现表明有潜力 体内 EPR 光谱研究顺磁代谢 化学和生化反应中发生的活性物质 小型和大型动物的皮肤，也可能是人类的皮肤。