STRUCTURE & FUNCTION ANALYSIS OF TOLEROGENIC PEPTIDES

结构

• 批准号: 6308890
• 负责人: JOHN H GRIFFIN
• 金额: $ 0.99万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-03-01 至 2002-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6308890
• 关键词: animal tissue; biological products; biomaterials; biomedical resource; drug /agent; human tissue; immunology; inflammation; lymphatic system; model design /development; spectrometry; structural biology; technology /technique; transplantation

We have undertaken a project in collaboration with colleagues in Stanford University School of Medicine to study peptides derived from MHC molecules which exhibit interesting immunomodulatory properties. The long-term goals of this effort are to understand in detail the basis for induction of T cell tolerization by these peptides, and also to develop potential candidates for use in human clinical immunsuppressive therapy. Our role is to carry out a structure/function analysis of MHC-derived peptides in order to determine the structural elements that are necessary and sufficient for immunosuppressive activity. To date, we have found, through a "serine scane" amino acid residues of a base sequence dodecapeptide which are critical for activity. One of these derivatives, and two other analogs have activity superior to that of the parent sequence. We plan to continue these studies, and to use tagged peptides as affinity ligands for receptor purification and biophysical characterization. FAB-MS is n invaluable tool for confirming the structure of the peptides and peptide derivatives that we synthesize in this

我们已经与同事合作进行了一个项目 斯坦福大学医学院研究源自 具有有趣的免疫调节特性的MHC分子。 这项努力的长期目标是详细了解 这些肽诱导T细胞耐受的基础，也是 开发潜在的候选人用于人类临床 免疫抑制治疗。 我们的角色是执行 MHC衍生肽的结构/功能分析以便 确定必要和足够的结构元素 用于免疫抑制活性。 迄今为止，我们已经通过 碱基序列十二肽的“丝氨酸Scane”氨基酸残基 这对于活动至关重要。 这些衍生物之一，两个 其他类似物具有优于父序的活性。 我们计划继续这些研究，并将标记的肽用作 亲和力配体用于受体纯化和生物物理 表征。 Fab-Ms是N的宝贵工具，可确认 我们合成的肽和肽衍生物的结构 在这个