STRUCT CHAR OF PROTEIN NUCLEIC ACID COMPLEXES IN NUCLEAR IMPORT

核输入中蛋白质核酸复合物的结构特征

• 批准号: 6308937
• 负责人: VITALY H CITOVSKY
• 金额: $ 0.97万
• 依托单位: BROOKHAVEN SCIENCE ASSOC-BROOKHAVEN LAB
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-04-01 至 2001-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6308937
• 关键词: bacteria; biomedical resource; genetics; growth /development; nucleic acids; plants; proteins

Nuclear import of proteins and nucleic acids is central to many cellular processes and cell-pathogen interactions. Some bacteria and viruses encode specialized proteins that Mansport genomic, DNA and RNA molecules of the invading pathogen into the host cell nucleus. One of these is the VirE2 protein of Agrobacterium. Agrobactenwn elicits neoplastic growths on many plant species by taiisporting a single-stranded copy of the bacterial tranderred DNA (T-DNA) into the. plant cell nucleus. VirE2 alone appears to be sufficient to transport T-DNA into the host cell nucleus. Since the T-DNA is not sequence specific, the complex between any ssDNA and VirE2 is competent for active nuclear imporL M 13 ssDNA and VirE2 were incubated and examined in the STEM. Both freeze-dried and negatively stained specimens were prepared. The rather rigid uniform filaments were analyzed, including mass/length, radial density profile analyses, and modeling to fit the observed stained data. A model of a hollow phone-cord like coil, 12.6 nin in diameter, with a helical pitch of 4.4 nrn, and approximately one VirE2 per twenty bases, fits all the data. TMV movement protein is not part of the TMV virion but is reponsible for the ftwisport into the host. Complexes of the protein and the TMV RNA were examined in the STEK but the protein/RNA ratio was too low. Further work is planned

蛋白质和核酸的核进口对于许多 细胞过程和细胞病原体相互作用。 一些细菌和 病毒编码Mansport基因组，DNA和RNA的专用蛋白质 入侵病原体的分子进入宿主细胞核。 之一 这些是农杆菌的VIRE2蛋白。 Agrobactenwn引起 泰伊斯波特（Taiisport） 细菌trander的DNA（T-DNA）的单链副本进入。 植物细胞核。 仅Vire2似乎就足够运输 T-DNA进入宿主细胞核。 由于T-DNA不是序列 具体而言，任何ssDNA和Vire2之间的复合物都有能力 活跃的核进口M 13 ssDNA和VIRE2孵育并检查 在茎中。 冷冻干燥和染色的标本都是 准备。 分析了相当刚性均匀的细丝 质量/长度，径向密度分析和建模以拟合 观察到的染色数据。 诸如COIL之类的空心电话型的模型，12.6 直径为NIN，螺旋螺距为4.4 NRN，大约 每二十个基地一个Vire2适合所有数据。 TMV运动蛋白 不是TMV病毒粒子的一部分 主人。 在中检查了蛋白质和TMV RNA的复合物 Stek，但蛋白质/RNA比太低。 进一步的工作是 计划