RAPID DETECTION OF TOXIC ALGAE USING UV RESONANCE RAMAN

使用紫外共振拉曼快速检测有毒藻类

• 批准号: 6314182
• 负责人: WILFRED NELSON
• 金额: $ 1.38万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-06-01 至 2001-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6314182
• 关键词: biological products; biomedical resource; infection; microorganism

The objective of this project to rapidly detect and identify toxin producing algae. Many types of algae are capable of producing toxins such as domoic acid. Since, domoic acid has well characterized UV visible absorption, UV resonance Raman spectroscopy can be used to selectively excite and observe domoic acid in algae. We have studied three kinds of algae Pseudonitzichia N95K, F996B, and V11B using 251 nm laser excitation. A series of standard domoic acid spectra were also obtained in the concentration range varying from 6 ppm to 96 ppb. Cross section of domoic acid 1640 cm-1 were also obtained by sodium sulfate as an internal standard.

该项目的目的快速检测和识别毒素 产生藻类。 许多类型的藻类能够产生毒素 例如多糖酸。 此后，多糖酸具有很好的紫外线 可见的吸收，紫外共振拉曼光谱法可用于 选择性地激发并观察到藻类中的多糖酸。 我们研究了 使用251 NM激光激发。 一系列标准的domoic酸光谱是 在浓度范围内获得的也从6 ppm到96 ppb不等。 还通过钠获得了多糖酸1640 cm-1的横截面 硫酸盐作为内标。